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本研究将PrP106-126多肽和HeLa细胞共孵育4h和8h,采用Hoechst染色分析发现PrP106-126诱导凋亡细胞细胞核出现不同程度的染色质浓集,固缩及碎裂的细胞凋亡征象。Western blotting检测发现PrP106-126诱导细胞中的多聚ADP核糖聚合酶(poly ADP-ribose polymerase,PARP)降解,提示PrP106-126通过caspase3途径引起细胞凋亡现象。PrP106-126诱导的细胞中14-3-3β在不同孵育时间也出现降解,而Real-time PCR检测14-3-3βmRNA未发生变化。本研究证明PrP106-126通过caspase3诱导HeLa细胞凋亡,并可导致抗凋亡蛋白14-3-3β的降解而加速凋亡的形成。
In this study, PrP106-126 polypeptide and HeLa cells were co-incubated for 4h and 8h. Hoechst staining showed that PrP106-126 induced apoptotic signs of chromatin condensation, pyknosis and fragmentation in apoptotic cells. Western blotting showed that PrP106-126 induced the degradation of poly ADP-ribose polymerase (PARP) in cells, suggesting that PrP106-126 induced apoptosis through caspase3 pathway. PrP106-126-induced cell 14-3-3β also degraded at different incubation times, whereas Real-time PCR did not show any change in 14-3-3β mRNA. This study demonstrated that PrP106-126 can induce the apoptosis of HeLa cells through caspase3 and lead to the degradation of anti-apoptotic protein 14-3-3β.