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目的:探讨应用电化学测菌法检测变形链球菌的可行性。方法:利用本课题组前期建立的电化学测菌法检测变形链球菌,记录检测参数,并将电化学测菌法的检测结果与细菌培养结果进行比较。结果:①微流芯片样本通道内层流形成时间为(3.17±0.16)min,从进样到获得稳定电阻抗数据的时间为(10.20±0.16)min。②当变形链球菌浓度为104～109cells/mL时,细菌浓度C的对数与系统阻抗值Z呈良好的线性关系(R2=0.98)。③与细菌培养的检测结果相比,电化学测菌法获得的细菌数多,差异有统计学意义(P<0.05)。④变形链球菌浓度为105cells/mL时,电化学测菌法检测值与细菌培养法检测值之间的线性函数关系式为:y=0.94x+0.32E4(R2=0.95),两种方法的检测值高度正相关(P=0.001<0.01)。结论:电化学测菌法能为变形链球菌的快速检测提供新的思路。
Objective: To explore the feasibility of electrochemical detection of Streptococcus mutans. Methods: The strain of Streptococcus mutans was detected by electrochemistry method and the parameters of the test were recorded. The results of electrochemical method were compared with the results of bacterial culture. Results: (1) The formation time of laminar flow in microfluidic chip was (3.17 ± 0.16) min, and the time from sampling to obtaining stable electrical impedance data was (10.20 ± 0.16) min. ② When the concentration of Streptococcus mutans was 104 ~ 109cells / mL, the logarithm of bacterial concentration C had a good linear relationship with the system impedance Z (R2 = 0.98). (3) Compared with the results of bacterial culture, the number of bacteria obtained by electrochemical method was significantly higher (P <0.05). ④ When the concentration of Streptococcus mutans was 105cells / mL, the linear function between the electrochemical detection method and the bacterial culture method was y = 0.94x + 0.32E4 (R2 = 0.95) The test values were highly correlated (P = 0.001 <0.01). Conclusion: Electrochemical method can provide a new idea for the rapid detection of Streptococcus mutans.