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目的： 观察氧化低密度脂蛋白（ｏｘＬＤＬ）对内皮细胞增殖与凋亡的影响以及抗氧化维生素（维生素Ｅ、维生素Ｃ及β－胡萝卜素）对内皮细胞的防治效应。方法： 在体外培养的小牛主动脉内皮细胞中分别加入不同浓度的抗氧化维生素，作用１２ｈ 后，再与终浓度为０．１ｇ Ｐｒ／Ｌ的ｏｘＬＤＬ共同培养２４ｈ，采用噻唑蓝比色分析法和流式细胞仪分析法对贴壁内皮细胞进行检测，分别观察抗氧化维生素对经ｏｘＬＤＬ作用的内皮细胞的形态、生长增殖、细胞周期及凋亡的影响。结果： （１）维生素Ｅ和维生素Ｃ能显著减轻ｏｘＬＤＬ对内皮细胞形态的损伤作用；β－胡萝卜素作用稍弱。（２）三种抗氧化维生素均可降低ｏｘＬＤＬ对内皮细胞生长增殖的抑制作用，使其抑制率降低。（３）三种抗氧化维生素均可促进内皮细胞由Ｇ１ 期进入ＤＮＡ合成的Ｓ期，促进内皮细胞的增殖并阻止内皮细胞凋亡的发生。结论： 维生素Ｅ、维生素Ｃ及β－胡萝卜素均可减轻ｏｘＬＤＬ在形态、增殖及凋亡等方面对内皮细胞的损伤作用，这可能是抗氧化维生素加速内皮细胞损伤后修复、促进内皮细胞增殖，从而发挥其抗动脉粥样硬化形成的部分机制。
AIM: To observe the effect of oxLDL on the proliferation and apoptosis of endothelial cells and the protective effect of antioxidant vitamins (vitamin E, vitamin C and β-carotene) on endothelial cells. Methods: Different concentrations of anti-oxidative vitamins were added to cultured aorta endothelial cells in vitro. After 12 hours of treatment, the cells were incubated with oxLDL at a final concentration of 0.1 g Pr / L for 24 hours. Thiazolyl blue colorimetry And flow cytometry analysis of adherent endothelial cells were detected antioxidant vitamins on oxLDL-induced endothelial cell morphology, growth and proliferation, cell cycle and apoptosis. Results: (1) Vitamin E and vitamin C can significantly reduce the damage of oxLDL to the morphology of endothelial cells; β-carotene weaker. (2) The three antioxidant vitamins can reduce oxLDL on endothelial cell growth and inhibition, so that inhibition rate decreased. (3) The three anti-oxidative vitamins can promote the endothelial cells to enter the S phase of DNA synthesis from G1 phase, promote the proliferation of endothelial cells and prevent the occurrence of endothelial cell apoptosis. CONCLUSION: Vitamin E, vitamin C and β-carotene can both reduce the effect of oxLDL on endothelial cell injury in morphology, proliferation and apoptosis. This may be due to the fact that antioxidant vitamins accelerate the repair of endothelial cells and promote endothelial cell proliferation, To play its part of anti-atherosclerosis mechanism.