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目的观察高盐摄入的小鼠循环单核细胞亚群的动态变化。方法雄性C57BL/6小鼠随机分为9 g/L Na Cl组、40 g/L Na Cl组和80 g/L Na Cl组。分别在处理前、处理后第4、8、12周,采用尾动脉测压法测定小鼠血压,高频小动物超声系统检测小鼠心功能,流式细胞术检测小鼠循环单核细胞亚群,心脏组织HE染色观察心脏病变情况。结果随着高盐饮食处理时间的延长,鼠尾收缩压呈现逐渐升高的趋势;与9 g/L Na Cl组相比,40 g/L Na Cl组和80 g/L Na Cl组小鼠射血分数(EF)呈先升高再降低的趋势,在第12周左室EF明显降低;40 g/L Na Cl组和80 g/L Na Cl小鼠循环淋巴细胞抗原6C高表达(Ly6C~(high))的单核细胞亚群比例均呈先升高再降低的趋势,在第12周时,降低明显;Ly6C~(low)单核细胞亚群的变化则与Ly6C~(high)单核细胞亚群呈相反的趋势,呈先降低再升高的趋势,在第12周时,明显升高;Ly6C~(int)单核细胞亚群无明显变化;心脏HE染色显示,与9 g/L Na Cl组相比,40 g/L Na Cl组和80 g/L Na Cl组心肌细胞直径明显变大。结论高盐摄入小鼠循环单核细胞亚群的比例发生动态变化。
Objective To observe the dynamic changes of circulating monocyte subsets induced by high salt in mice. Methods Male C57BL / 6 mice were randomly divided into 9 g / L NaCl group, 40 g / L NaCl group and 80 g / L NaCl group. The blood pressure of mice was measured by tail arterial manometry at 4, 8 and 12 weeks after treatment, respectively. The heart function of mice was detected by high frequency small animal ultrasound system. The levels of circulating monocyte Group, heart tissue HE staining observed heart disease. Results Compared with 9 g / L NaCl group, 40 g / L NaCl group and 80 g / L NaCl group showed higher systolic blood pressure (SBP) The ejection fraction (EF) increased first and then decreased, and the left ventricular ejection fraction decreased significantly at the 12th week. The circulating lymphocyte antigen 6C was highly expressed in the 40 g / L NaCl group and 80 g / L NaCl mice (Ly6C ~ (high)) monocytes increased first and then decreased, and decreased significantly at the 12th week. The changes of Ly6C ~ (low) monocyte subsets were similar to Ly6C ~ (high) Monocyte subsets showed the opposite trend, first decreased and then increased, at the twelfth week, increased significantly; Ly6C ~ (int) monocyte subsets no significant change; HE staining showed that 9 Compared with g / L Na Cl group, the diameters of cardiomyocytes in 40 g / L NaCl group and 80 g / L NaCl group were significantly larger. Conclusion The proportion of circulating monocyte subsets in mice with high salt intake changes dynamically.