论文部分内容阅读
目的获得可以用于B病毒检测的gB蛋白特异性表位重组抗原。方法利用基因合成的方法,合成包含B病毒gB蛋白主要特性抗原表位的基因,通过定向克隆的方法,将该基因克隆到原核表达载体pET-22b中,构建了重组表达质粒。筛选出阳性重组质粒,转化到BL21感受态细胞,利用IPTG进行诱导表达。结果成功的获得了B病毒gB蛋白的特异性抗原蛋白,该蛋白以可溶的形式表达。结论利用原核表达系统,可以产生B病毒gB蛋白特异性表位重组抗原,可以作为B病毒的检测抗原。
Objective To obtain gB protein specific epitope recombinant antigen that can be used for B virus detection. Methods Genes containing antigenic epitopes of gB protein of B virus were synthesized by gene synthesis. The gene was cloned into prokaryotic expression vector pET-22b by directional cloning, and a recombinant plasmid was constructed. Positive recombinant plasmids were screened and transformed into BL21 competent cells, which were induced by IPTG. As a result, the specific antigen protein of the B virus gB protein was successfully obtained, and the protein was expressed in a soluble form. Conclusion The prokaryotic expression system can produce gB protein specific epitope recombinant antigen of B virus, which can be used as detection antigen of B virus.