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目的：探讨蛋白激酶Ｃ（ＰＫＣ）激活参与神经元损伤的机理。方法：采用新生Ｗｉｓｔａｒ大鼠神经元分散培养，观察ＰＫＣ激动剂ＰＭＡ对培养神经元的毒性率及神经元内游离钠的影响。结果：ＰＭＡ可以明显地增加细胞内游离钙和神经元的毒性率。结论：ＰＫＣ激活引起神经元损伤可能是通过引起钙聚积来实现的
Objective: To investigate the mechanism of protein kinase C (PKC) activation involved in neuronal injury. Methods: The cultured neonatal Wistar rat neurons were cultured in vitro to observe the effects of PMA, a PKC agonist, on the neurotoxicity and the intracellular free sodium in neurons. Results: PMA significantly increased the intracellular free calcium and neuronal toxicity. Conclusion: PKC activation induced neuronal damage may be caused by the accumulation of calcium to achieve