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目的 研究依托咪酯对大鼠脑ｃＡＭＰ含量及腺苷酸环化酶（ＡＣ） 活性的影响。方法 ３０ 只ＳＤ 大鼠，随机分为３ 组，即对照组、依托咪酯１ 组和依托咪酯２ 组。对照组等容腹腔注射生理盐水５ ｍｌ／ｋｇ，依托咪酯１ 和２组分别腹腔注射依托咪酯６ ｍｇ／ｋｇ 和１２ ｍｇ／ｋｇ。前２ 组腹腔注射３ ｍｉｎ 后断头，后一组翻正反射消失后立即断头。分别采用蛋白竞争结合法及以非标记ＡＴＰ为底物的方法测ＳＤ大鼠各脑区（ 皮质、海马、脑干）的ｃＡＭＰ含量与ＡＣ活性变化。结果 依托咪酯１ 组大鼠行为变迟钝，依托咪酯２ 组大鼠翻正反射消失、皮层内ｃＡＭＰ含量升高且与对照组相比以及两剂量组间皆存在统计学差异（ Ｐ＜ ０．０５）。两组海马ｃＡＭＰ含量均高于对照组（ Ｐ＜０ ．０５）。依托咪酯对各脑区ＡＣ活性变化无明显影响（ Ｐ ＞ ０．０５）。结论 依托咪酯的全身麻醉作用与其升高皮质、海马ｃＡＭＰ的含量有关。
Objective To investigate the effects of etomidate on cAMP content and adenylyl cyclase (AC) activity in rat brain. Methods Thirty SD rats were randomly divided into 3 groups: control group, etomidate group 1 and etomidate group 2. The control group was intraperitoneally injected with normal saline 5 ml / kg, etomidate 1 and 2 groups were injected ip with etomidate 6 mg / kg and 12 mg / kg, respectively. Before the first two groups, 3 min after intraperitoneal injection, the decapitated group immediately after decapitation disappeared. The changes of cAMP content and AC activity in various brain regions (cortex, hippocampus and brainstem) of SD rats were measured by protein competition binding assay and non-labeled ATP as substrate. Results The behavior of rats in etomidate group 1 became dull. The reflex of etomidate group 2 disappeared. The content of cAMP in the cortex increased significantly compared with the control group and the two dose groups (P <0. .05). The cAMP content in hippocampus in both groups were higher than that in control group (P <0.05). Etomidate had no significant effect on AC activity in various brain regions (P> 0.05). Conclusion The general anesthetic effect of etomidate is related to the increase of cAMP content in cortex and hippocampus.