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将亚洲栽培稻(Oryza sativa,染色体组型为AA)与宽叶野生稻(O. latifolia,染色体组型为CCDD)进行杂交,结合胚拯救手段,获得了这两个种的种间杂交种.杂种F1在株高、分蘖力、生长繁茂性等方面表现明显的杂种优势,穗部性状明显偏向于父本宽叶野生稻的特征,表现为长芒、小粒、大而外露的紫色柱头极易落粒.根尖细胞染色体鉴定证明杂种染色体数为2n=36.进一步利用基因组原位杂交(genomic in situ hybridization,GISH)和荧光原位杂交(fluorescent in situ hybridization,FISH)技术,鉴定了杂种F1的染色体组成及其在减数分裂中的配对行为.结果表明,杂种染色体的组成为ACD,在减数分裂中,绝大部分染色体以单价体形式存在,很少有配对现象发生,因而杂种表现完全雄性不育。
The cross between two cultivars of Oryza sativa (genome type AA) and O. latifolia (chromosome type CCDD) was used in combination with embryo rescue methods. Hybrid F1 showed obvious heterosis in plant height, tillering ability, growth and lushness, etc. The panicle traits were obviously biased toward the characteristics of male wild broad-leaved rice, which showed that the purple capitula was easy to grow. The number of chromosomes in root tip cells was 2n = 36. The genomic in situ hybridization (GISH) and fluorescence in situ hybridization (FISH) were used to identify the hybrid F1 And its pairing behavior in meiosis.The results showed that the composition of the hybrid chromosome was ACD, most of the chromosomes existed as monovalent forms in meiosis, and few pairs of phenotypes appeared, so hybrid performance Completely male sterile.