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目的:探讨在高转移人乳腺癌细胞中,核因子κB活性对肿瘤生长及运动迁移能力的影响。方法:转染显性负性突变的ⅠκBα重组质粒入高转移乳腺癌细胞MDA-MB-231、MDA-MB-435,筛选并鉴定稳定转染的细胞株;凝胶迁移实验观察核因子κB活性,细胞生长曲线、平板集落形成试验及Millicell-PCF培养小室观察质粒转染对细胞生长和趋化运动的影响。结果:乳腺癌MDA-MB-231、MDA-MB-435细胞中存在高NF-κB组成型激活,稳定转染显性负性的ⅠκBα质粒后,细胞NF-κB活性下调,在不明显影响细胞生长、克隆形成的情况下,抑制高转移乳腺癌细胞株的运动能力。结论:在体外,抑制NF-κB通路,可明显抑制高转移乳腺癌细胞株的运动迁移能力。
Objective: To investigate the effect of nuclear factor κB activity on tumor growth and motility in highly metastatic human breast cancer cells. Methods: Transfect the negative mutant ⅠκBα into the highly metastatic breast cancer cells MDA-MB-231 and MDA-MB-435 to screen and identify stable transfected cell lines. The activity of nuclear factor κB , Cell growth curve, plate colony formation assay and Millicell-PCF culture chamber to observe the effect of plasmid transfection on cell growth and chemotaxis. Results: The constitutive activation of NF-κB in breast cancer MDA-MB-231 and MDA-MB-435 cells was constitutively activated. Stably transfected with negative IκBα plasmid, the NF-κB activity was down- Growth, clonogenicity, suppresses motility of highly metastatic breast cancer cell lines. CONCLUSION: In vitro, inhibition of NF-κB pathway can significantly inhibit the migration of highly metastatic breast cancer cell lines.