详细讨论了用异硫氰酸苯酯（ＰＩＴＣ）柱前衍生，反相色谱法测定氨基酸（即ＰＩＣＯ－ＴＡＧ法）的最佳条件。研究了流动相配制时溶液ｐＨ值、乙腈含量等对色谱分析的影响，并且对色谱柱的退化与再生进行了说明。氨基酸含量在１．２５～１５００ｐｍｏｌ范围内线性良好，相关系数大于０．９９９，且大多数氨基酸保留时间的变异系数小于０．５％。氨基酸采用峰面积定量，测定变异系数为０．１～１．５％。 The optimal conditions for the determination of amino acids (ie, PICO-TAG method) by column derivatization with Phenylisothiocyanate (PITC) and reverse phase chromatography were discussed in detail. The influence of pH and acetonitrile content on the chromatographic analysis of the mobile phase was studied. The degradation and regeneration of the column were described. Amino acid contents ranged from 1.25 to 1500 pmol with good linearity, a correlation coefficient greater than 0.999, and a coefficient of variation for most amino acid retention times of less than 0.5%. Amino acids were quantified by peak area, and the coefficient of variation was determined to be 0.1-1.5%.