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目的探讨生物波调控因子(Bio-wave regulation factor,BRF)对大鼠局灶性脑缺血损伤后行为学及脑组织内肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)表达的影响。方法成年健康雄性SD大鼠90只随机分为3组,即假手术组、生理盐水组、BRF治疗组,每组分为6、24、48、72h、7d,5个亚组,每亚组6只大鼠。参照Longa等的线栓法制成大脑中动脉闭塞(middle cerebral artery occlusion,MCAO)模型,术后1h以1ml/100g体重的剂量分别腹腔注射1.25%BRF溶液和生理盐水,此后1次/d重复注射,至相应时间点处死动物。采用Longa等4种方法进行行为学评分、干湿重法测定脑组织含水量、HE染色观察组织病理学改变、免疫组织化学方法观察脑组织TNF-α的动态变化。结果1.行为学评分:MCAO大鼠对侧肢体存在不同程度瘫痪,在术后48h和72h神经功能缺损最为明显,7d基本恢复正常;2.脑组织含水量测定:脑梗死组脑组织含水量在各时间段均增加,其中术后48hBRF组低于同期的生理盐水组(P<0.05);3.脑组织HE染色观察:除假手术组外,其余各组在脑梗死后6h局部可见炎性细胞浸润,48h明显增多,并持续到7d;4.脑组织TNF-α的表达:与假手术组相比,脑梗死组TNF-α阳性细胞在各时间段均增加,术后48h、72hBRF组低于同期的生理盐水组(P<0.05)。结论BRF通过减轻脑水肿,降低脑内肿瘤坏死因子-α的表达,对大鼠缺血脑组织产生保护作用。
Objective To investigate the effects of bio-wave regulation factor (BRF) on the behavior and the expression of tumor necrosis factor-α (TNF-α) in brain tissue after focal cerebral ischemia in rats influences. Methods Ninety adult male Sprague-Dawley rats were randomly divided into 3 groups: sham operation group, saline group and BRF treatment group, each group was divided into 6, 24, 48, 72h, 7d, 5 subgroups, 6 rats. The middle cerebral artery occlusion (MCAO) model was made by the method of Longa et al. The rats were injected intraperitoneally with 1.25% BRF solution and normal saline at a dose of 1 ml / 100 g at 1 hour after operation, and then injected once / d repeatedly Animals were sacrificed at the corresponding time points. Behavioral score was measured by Longa et al. Four methods were used to measure the brain water content. The histopathological changes were observed by HE staining. The dynamic changes of TNF-α in brain tissue were observed by immunohistochemistry. Results 1. Behavioral score: The contralateral limbs of MCAO rats were paralyzed to varying degrees, the neurological deficits were most obvious at 48h and 72h after operation, and returned to normal after 7d. 2. The determination of brain water content: The brain water content (P <0.05); (3) The brain tissue HE staining: except the sham operation group, the other groups in the 6h after cerebral infarction local inflammation The number of TNF-α positive cells in cerebral infarction group increased at each time period, and the expression of TNF-α in brain tissue increased at 48h and 72h Group below the same period of saline group (P <0.05). Conclusion BRF can protect cerebral ischemia in rats by relieving cerebral edema and decreasing the expression of tumor necrosis factor-α in the brain.