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目的:探讨川芎嗪(TMP)预处理骨髓间充质干细胞(BMSCs)移植对脑缺血大鼠神经细胞凋亡及Bcl-2、Bax表达的影响。方法:采用全骨髓贴壁法分离BMSCs,川芎嗪(50、100μmol/L)预处理24h。线栓法诱导大鼠大脑中动脉阻塞模型,分假手术组、模型组、BMSCs组和川芎嗪预处理组。缺血后第1、7和14天,采用改良的神经损伤严重程度评分(m NSS)和角试验进行神经功能评价;缺血后第14天,采用原位末端标记(TUNEL)法观察神经细胞凋亡,Western blot法检测Bcl-2和Bax蛋白的表达。结果:与BMSCs组比较,川芎嗪预处理组大鼠m NSS评分和右转次数均显著减少,TUNEL阳性细胞数显著减少(P<0.05,P<0.01),Bcl-2蛋白表达显著上调,Bax蛋白表达显著下调(P<0.05,P<0.01)。结论:川芎嗪预处理BMSCs移植促进脑缺血大鼠神经功能恢复,机制可能与其调控Bcl-2/Bax表达抑制神经细胞凋亡有关。
Objective: To investigate the effects of ligustrazine (TMP) pretreatment of bone marrow mesenchymal stem cells (BMSCs) transplantation on neuronal apoptosis and expression of Bcl-2 and Bax in cerebral ischemia rats. Methods: BMSCs were isolated by whole bone marrow adherent method and ligustrazine (50,100μmol / L) for 24 hours. The model of middle cerebral artery occlusion was induced by thread occlusion in rats. The rats were divided into sham operation group, model group, BMSCs group and ligustrazine preconditioning group. On the 1st, 7th and 14th day after ischemia, the neurological function was assessed by m NSS and angle test. On the 14th day after ischemia, neurons were observed by TUNEL The apoptosis and Western blot were used to detect the expression of Bcl-2 and Bax protein. Results: Compared with the BMSCs group, the m NSS score and the number of right turn of rats in the Ligustrazine preconditioning group were significantly decreased, the number of TUNEL positive cells was significantly decreased (P <0.05, P <0.01), Bcl-2 protein expression was significantly increased, Bax Protein expression was significantly down-regulated (P <0.05, P <0.01). CONCLUSION: Tetramethylpyrazine preconditioning with BMSCs can promote the recovery of neural function after focal cerebral ischemia in rats. The mechanism may be related to the regulation of Bcl-2 / Bax expression and the inhibition of neuronal apoptosis.