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通过Pubmed和CNKI数据库查阅了近几年应用离体肾灌流(isolated perfused kidney,IPK)技术在药理毒理研究方面的文献,并进行了整理和分析。IPK技术的主要特点是一定时间内接近体内肾的外环境,通过不同时间点从输尿管和肾静脉流出的灌流液中采样,动态观察外源性物质进入肾脏所发生的变化。IPK技术可用于研究药物在肾脏的排泄过程、作用机制以及药物的联用等。此外,通过检测灌流液中蛋白质活性及含量的变化,结合肾组织病理学变化,还可用于研究药物对肾损伤特点、程度、机制等。该文对离体肾灌流的技术特点及其在药理毒理研究中的应用进行介绍,以供药物研究和临床应用参考。
The Pubmed and CNKI databases were used to review the literature on pharmacology and toxicology using isolated perfused kidney (IPK) technology in recent years and to organize and analyze them. The main feature of IPK technology is that it is close to the external environment of the kidney in a certain period of time and sampled from the perfusate flowing out from the ureter and renal vein at different time points to dynamically observe the changes of exogenous substances into the kidney. IPK technology can be used to study the excretion of drugs in the kidneys, the mechanism of action and the combination of drugs. In addition, by detecting changes in protein activity and content of perfusate, combined with renal histopathological changes, can also be used to study the characteristics of the drug on renal injury, extent, mechanism. In this paper, the technical characteristics of isolated renal perfusion and its application in the study of pharmacology and toxicology are introduced for the reference of drug research and clinical application.