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目的探讨芎冰微乳对脑缺血再灌注损伤的保护作用及其作用机制。方法采用线栓法制作大鼠局灶性脑缺血-再灌注模型及小鼠双侧颈总动脉夹闭的脑缺血再灌注模型。观察芎冰微乳对脑组织含水量、大鼠脑梗死面积、脑组织中超氧化物歧化酶(MDA)、脂质过氧化物丙二醛(SOD)、一氧化氮(NO)含量变化的影响。结果与模型组相比,芎冰微乳组脑组织含水量、梗死面积显著减小,脑组织中MDA、NO的含量显著降低,SOD的活性显著提高,上述作用与尼莫地平对照组相似。结论芎冰微乳对大鼠、小鼠脑缺血再灌注损伤具有保护作用,其机制可能与抑制NO合成,提高SOD的活性,减少脂质过氧化作用有关。
Objective To investigate the protective effect of Xiong Bing microemulsion on cerebral ischemia-reperfusion injury and its mechanism. Methods Focal cerebral ischemia - reperfusion model and cerebral ischemia - reperfusion model of bilateral common carotid artery occlusion in rats were made by thread plug method. To observe the effect of Xiongxiong microemulsion on the changes of water content in brain tissue, infarct size, superoxide dismutase (MDA), malondialdehyde (MDA) and nitric oxide (NO) . Results Compared with the model group, the water content and the infarct size in the Xiong Bing microemulsion group decreased significantly. The content of MDA and NO in the brain tissue decreased significantly and the activity of SOD increased significantly. The effect was similar to that in the nimodipine control group. Conclusion Xiong Bing microemulsion has a protective effect on cerebral ischemia-reperfusion injury in rats and mice. The mechanism may be related to inhibition of NO synthesis, increase of SOD activity and reduction of lipid peroxidation.