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为了探讨甲基紫精(MV)对丹参(Salvia miltiorrhiza)体内抗氧化防护系统的影响及其生理机制。以MV为诱导剂,以敌草隆(DCMU)为抑制剂,考察了MV与DCMU处理后丹参悬浮培养细胞中H2O2、丙二醛、还原型谷胱甘肽的含量以及抗氧化防护酶(超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT))活性变化和同工酶的表达差异。结果表明,MV处理显著提高了丹参培养细胞内H2O2、丙二醛以及还原型谷胱甘肽含量;MV处理使CAT、POD活性增强,谱带颜色更亮,条带增加。DCMU处理显著抑制了MV诱导的H2O2、丙二醛、还原型谷胱甘肽含量的增加,抗氧化酶活性的升高和同工酶的表达。以上结果说明,MV可诱导丹参培养细胞叶绿体产生H2O2,H2O2激活了丹参培养细胞抗氧化防护系统以维持细胞正常的生理活动。
To investigate the effect of methyl viologen (MV) on the anti-oxidant protective system in Salvia miltiorrhiza and its physiological mechanism. Using MV as inducer and diuron (DCMU) as inhibitor, the contents of H2O2, malondialdehyde and reduced glutathione (GSH) and the content of antioxidant enzymes (SOD), peroxidase (POD), catalase (CAT)) activity and isozyme expression differences. The results showed that MV treatment significantly increased H2O2, malondialdehyde and reduced glutathione content in cultured Salvia miltiorrhiza cells. MV treatment enhanced CAT, POD activity, brighter bands and increased bands. DCMU treatment significantly inhibited the MV induced H2O2, malondialdehyde, reduced glutathione content, antioxidant enzyme activity and isozyme expression. The above results show that MV can induce H2O2 production in the chloroplasts of Salvia miltiorrhiza cultured cells, and H2O2 activates the antioxidant defense system of Salvia miltiorrhiza to maintain the normal physiological activities of cells.