论文部分内容阅读
目的探讨女贞子中乙酰齐墩果酸、齐墩果酸、19α-羟基-3-乙酰乌索酸、槲皮素、GI3、女贞苷、对羟基苯乙醇-O--βD-葡萄糖苷、芹菜素-6″-O乙酰-7-O-β-D-葡萄糖苷对胰岛素抵抗HepG2细胞葡萄糖消耗的影响。方法采用高浓度胰岛素诱导培养HepG2细胞,建立胰岛素抵抗细胞模型,培养液中加入各化合物共同孵育,采用葡萄糖试剂盒检测培养液中的葡萄糖含量,探讨其对胰岛素抵抗HepG2细胞葡萄糖消耗的影响。结果齐墩果酸、19α-羟基-3-乙酰乌索酸、GI3、对羟基苯乙醇-O-β-D-葡萄糖苷、芹菜素-6″-O-乙酰-7-O-β-D-葡萄糖苷在不含胰岛素条件下可使胰岛素抵抗HepG2细胞的葡萄糖消耗量增加11.62%、17.21%、10.54%、12.08%、11.85%,加入生理浓度胰岛素后,上述化合物可使细胞葡萄糖消耗量增加12.95%、13.83%、10.84%、14.26%、17.41%。槲皮素、女贞苷在不含胰岛素条件下不增加细胞的葡萄糖消耗量,加入生理浓度胰岛素后,可使细胞葡萄糖消耗量增加15.70%、17.04%,其中槲皮素促进细胞增殖。结论齐墩果酸、芹菜素-6″-O-乙酰-7-O--βD-葡萄糖苷等增加胰岛素抵抗HepG2细胞的葡萄糖消耗量呈非胰岛素依赖性,其中芹菜素-6-″O-乙酰-7-O--βD-葡萄糖苷与生理浓度胰岛素有协同增强作用;女贞苷、槲皮素增加细胞葡萄糖消耗量呈胰岛素依赖性,槲皮素能显著促进细胞增殖。
OBJECTIVE To investigate the effects of oleanolic acid, oleanolic acid, 19α-hydroxy-3-acetoxulohilic acid, quercetin, GI3, virginia glycosides and p-hydroxyphenethyl alcohol O-β D-glucoside , And the effect of apigenin-6 “-O-acetyl-7-O-β-D-glucoside on HepG2 cell insulin resistance.Methods HepG2 cells were induced by high concentration of insulin and established a model of insulin resistance cells, The compounds were incubated together, and the glucose content in the culture medium was detected by glucose kit to investigate the effect of insulin on the glucose consumption of HepG2 cells.Results Oleanolic acid, 19α-hydroxy-3-acetoxamate, GI3, Phenylethanol-O-β-D-glucoside and apigenin-6 ”-O-acetyl-7-O-β-D-glucoside increased glucose consumption of insulin resistance HepG2 cells in the absence of insulin by 11.62 %, 17.21%, 10.54%, 12.08% and 11.85%, respectively. After adding physiological concentration of insulin, the above compounds could increase cell glucose consumption by 12.95%, 13.83%, 10.84%, 14.26% and 17.41% respectively. Quercetin and virginia glycosides did not increase the glucose consumption of cells in the absence of insulin. Glucose consumption increased by 15.70% and 17.04%, respectively, after adding physiological concentrations of insulin, of which quercetin promoted cell proliferation. Conclusion The glucose consumption of HepG2 cells with increased insulin resistance such as oleanolic acid and apigenin-6 "-O-acetyl-7-O-β-D-glucoside is non-insulin-dependent. Acetyl-7-O-β-glucosidase and synergistic enhancement of physiological concentrations of insulin; Ligustrazine, quercetin increased cell glucose consumption was insulin-dependent, quercetin can significantly promote cell proliferation.