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目的 研究不同含锌饲料对生长大鼠学习记忆的影响及与其脑中生长抑素、锌和钙含量的关系 ,以确定维持大鼠最佳学习记忆状态的补锌范围。方法 以含锌量为 2 0、50、1 0 0、2 0 0、40 0和 80 0 mg/kg的饲料饲喂 88g左右的 Wistar大鼠 ,雌雄各半 ;以避暗法测试各组实验动物学习记忆能力 ,并以放免法测定了脑组织生长抑素 ;以原子吸收光谱法测定脑组织锌和钙含量。结果 1 0 0 mg/kg和 2 0 0 mg/kg的饲料锌含量 ,使大鼠学习记忆功能最好 ,而较低和较高的饲料锌水平对学习记忆功能均有不同程度的不利影响 ;同时较低和较高的饲料锌水平使下丘脑、海马和大脑皮层生长抑素水平都有不同程度的降低 ;另外 ,较低的饲料锌水平可使血液锌、海马、大脑皮层和下丘脑锌水平一致性地降低 ,同时使大脑皮层钙含量降低。结论 1 0 0～ 2 0 0 mg/kg饲料锌含量 ,对维持大鼠学习记忆功能是最适的补锌范围 ,这与大鼠脑组织较高的生长抑素、锌和钙水平一致。
Objective To study the effect of different zinc-containing diets on the learning and memory of growing rats and its relationship with the content of somatostatin, zinc and calcium in brain so as to determine the range of zinc supplementation for maintaining the best learning and memory state in rats. Methods Wistar rats weighing about 88 g were fed with diets containing 20, 50, 100, 200, 40 and 80 mg / kg of zinc, Animal learning and memory ability, and somatostatin was determined by radioimmunoassay. The contents of zinc and calcium in brain tissue were determined by atomic absorption spectrometry. Results Dietary Zn levels of 1 0 0 mg / kg and 200 mg / kg of diet significantly improved learning and memory in rats, whereas lower and higher levels of dietary zinc had different effects on learning and memory. At the same time, lower and higher dietary zinc levels decreased the levels of somatostatin in the hypothalamus, hippocampus and cerebral cortex. In addition, lower levels of dietary zinc could make zinc, hippocampus, cerebral cortex and hypothalamus zinc Reduce the level of consistency, while reducing the cerebral cortex calcium content. Conclusion The dietary zinc content of 100 ~ 200 mg / kg diet is the most suitable zinc supplement for the maintenance of learning and memory in rats, which is consistent with the higher somatostatin, zinc and calcium levels in rat brain.