Objective:To assess the antibacterial activity of eight Iranian plant extracts against Staphylococcus aureus(S.aureus) strains which were isolated from infected patients.Methods: The studied strains were isolated from urine,stool,blood and wound of infected patients and identified by biochemical tests.In further,the antibacterial activity of 8 ethanolic local plant extracts including Querqus brantii(Q.brantii),Ziziphus spina-christi(Z.spina-christi),Peganum harmala(P.harmala),Oliveira decumbens(O.decumbens),Galium tricornutum(G.tricornutum), Vitex pseudo negundo(Vi.pseudo negundo),Salvia officinalis(S.officinalis),Vaccaria pyramidata (V.pyramidata) were then evaluated using agar disc diffusion method.Results:A total of 9 isolates were isolated and identified as S.aureus.Antibacterial profile of the strains showed that the strains were resistant to methicillin and cefixime.The highest antibacterial activity against the studied strains were belong to Q.brantii,P.harmala,Z.spina-christi and O.decumbens vent extracts with 11-40 mm,15-28 mm,8-26 mm and 10-20 mm of diameters,respectively. Intermediate antibacterial activity was exhibited by G.tricornutum and Vi.pseudo negundo against some of the studied strains with 7-20 mm and 7-13 mm of diameters,respectively. Noteworthy,out of 9 studied strains;6 and 2 of them were resistant to the G.tricornutum and Vi. pseudo negundo extracts,respectively.The S.officinalis and Va.pyramidata,however,showed no antibacterial activity against the studied strains.Conclusions:The Q.brantii,P.harmala,Z. spina-christi and O.decumbens extracts were shown to possess strong antibacterial activity against methicillin and cefixime resistant S.aureus strains and can be considered as the promising natural antibiotics for treating the studied strains. Objective: To assess the antibacterial activity of eight Iranian plant extracts against Staphylococcus aureus (S. aureus) strains which were isolated from infected patients. Methods: The studied strains were isolated from urine, stool, blood and wound of infected patients and identified by biochemical tests.In addition, the antibacterial activity of 8 ethanolic local plant extracts including Querqus brantii (Q. brantii), Ziziphus spina-christi (Z. spina-christi), Peganum harmala (P. harmala), Oliveira decumbens (O. decumbens) , Vitex pseudo negundo (Vi.pseudo negundo), Salvia officinalis (S. officinalis), Vaccaria pyramidata (V. pyramidata) were then evaluated using agar disc diffusion method. Results: A total of 9 isolates were isolated and identified as S. aureus. An antibacterial profile of the strains showed that the were were resistant to methicillin and cefixime. highest highest in an activity of the AIDS against the studied. were were belong to Q.brantii, P.harmala, Z. spina-christi nd O. decumbens vent extracts with 11-40 mm, 15-28 mm, 8-26 mm and 10-20 mm of diameters, respectively. Intermediate antibacterial activity was indicated by G.tricornutum and Vi.pseudo negundo against some of the studied Noteworthy, out of 9 studied; 6 and 2 of them were resistant to the G.tricornutum and Vi. pseudo negundo extracts, respectively.The S. officinalis and Va.pyramidata, however, showed no antibacterial activity against the studied strain. Conclusions: The Q. brantii, P. harmala, Z. spina-christi and O. decumbens extracts were shown to possess strong antibacterial activity activity against methicillin and cefixime resistant S. aureus strains and can be considered as the promising natural antibiotics for treating the studied strains.