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目的:观察豚鼠变应性鼻炎动物模型的鼻腔灌洗液(NLF)中血栓素(TX)B2和白三烯(LTs)水平及地氯雷他定对这些递质的影响。方法:将36只豚鼠分为阳性对照组、地氯雷他定(desloratadine)治疗组和阴性对照组,每组12只。阴性对照组未予抗原致敏和激发,阳性对照组和地氯雷他定治疗组经卵白蛋白局部致敏,卵白蛋白溶液反复局部激发后,收集并测定各组NLF中TXB2和LTs水平。结果:NLF中,阳性对照组TXB2水平[(271.8±36.5)pg/1mlNLF]较地氯雷他定治疗组[(156.4±34.0)pg/1mlNLF]及阴性对照组[(62.1±16.0)pg/1mlNLF]高,差异均有统计学意义(均P<0.01);地氯雷他定治疗组较阴性对照组高(P<0.01)。阳性对照组LTs水平[(114.5±40.0)pg/1mlNLF]较地氯雷他定治疗组[(66.0±16.7)pg/1mlNLF]及阴性对照组[(12.4±2.3)pg/1mlNLF]高,差异均有统计学意义(P<0.05和P<0.01);地氯雷他定治疗组较阴性对照组高(P<0.05)。结论:在豚鼠变应性鼻炎动物模型中,其NLF中的TXB2和LTs水平在抗原激发后显著升高;地氯雷他定能显著抑制TXB2和LTs水平,提示地氯雷他定的治疗机制与抑制TXB2和LTs的产生或释放有关。
Objective: To observe the levels of thromboxane B2 and leukotrienes (LTs) in nasal lavage fluid (NLF) of guinea pigs with allergic rhinitis and the effect of desloratadine on these neurotransmitters. Methods: Thirty-six guinea pigs were divided into positive control group, desloratadine treatment group and negative control group, 12 rats in each group. The negative control group was not sensitized and challenged by antigen. Positive control group and desloratadine treatment group were orally induced by ovalbumin. After repeated local challenge with ovalbumin solution, the levels of TXB2 and LTs in each group were collected and measured. Results: In NLF, the level of TXB2 in the positive control group [(271.8 ± 36.5) pg / 1mlNLF] was significantly lower than that in the desloratadine treatment group [(156.4 ± 34.0) pg / 1mlNLF] and the negative control group (62.1 ± 16.0pg / 1mlNLF], the differences were statistically significant (all P <0.01); desloratadine treatment group than the negative control group (P <0.01). The level of LTs in the positive control group [(114.5 ± 40.0) pg / 1mlNLF] was significantly higher than that in the desloratadine treatment group (66.0 ± 16.7 pg / 1mlNLF) and the negative control group (12.4 ± 2.3 pg / 1mlNLF) (P <0.05 and P <0.01). The desloratadine treatment group was higher than the negative control group (P <0.05). CONCLUSIONS: In the animal model of allergic rhinitis in guinea pigs, the level of TXB2 and LTs in NLF significantly increased after antigen challenge; desloratadine significantly inhibited the levels of TXB2 and LTs, suggesting the therapeutic mechanism of desloratadine Related to the inhibition of TXB2 and LTs production or release.