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目的观察不同时间给予己酮可可碱(pentoxifylline,PTX)对新生大鼠缺氧缺血性脑损伤(hypoxic-ischemic brain damage,HIBD)模型的影响。方法建立新生大鼠HIBD模型,120只出生7 d的wistar大鼠随机分为假手术(Sham)组、HIBD模型组和3种PTX(50 mg／kg腹腔注射)给药组,即缺血末均给药(I+PTX)组、缺氧末给药(H+PTX)组及缺血和缺氧末均给药(I,H+PTX)组,各组均于缺血缺氧处理后72 h取出左脑,测定脑组织水含量、大脑皮层神经组织游离钙离子浓度、超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮合酶(NOS)及一氧化氮(NO)。结果HIBD模型组与Sham组相比,脑组织含水量、游离钙离子浓度、MDA、NOS及NO水平显著升高(均P＜0.01),SOD水平明显降低(P＜0.01)。3种PTX给药组与HIBD模型组相比,脑组织含水量、游离钙离子浓度、MDA、NOS及NO水平均显著降低(均P＜0.01),SOD水平明显升高(P＜0.01)。以上作用尤以(I,H+PTX)组明显。结论 PTX对新生大鼠缺氧缺血后脑组织有保护作用,其机制可能与PTX改善能量代谢及减少局部细胞毒性物质有关。
Objective To observe the effects of pentoxifylline (PTX) on hypoxic-ischemic brain damage (HIBD) in neonatal rats at different time points. Methods The HIBD model of neonatal rats was established. 120 wistar rats born at 7 days were randomly divided into Sham group, HIBD model group and three PTX groups (50 mg / kg intraperitoneal injection) (I + PTX), hypoxia (H + PTX) and ischemia and hypoxia (I, H + PTX) The left brain was removed at 72 h, and the water content in brain tissue, free calcium concentration in brain tissue, superoxide dismutase (SOD), malondialdehyde (MDA), nitric oxide synthase (NOS) and nitric oxide NO). Results Compared with Sham group, the water content, free calcium concentration, MDA, NOS and NO in HIBD model group were significantly increased (all P <0.01) and SOD levels were significantly decreased (P <0.01). Compared with HIBD model group, the water content, free calcium concentration, MDA, NOS and NO in three PTX groups were significantly decreased (all P <0.01) and SOD levels were significantly increased (P <0.01). Above effect (I, H + PTX) group is obvious. Conclusion PTX can protect brain tissue of neonatal rats after hypoxia-ischemia. Its mechanism may be related to the improvement of energy metabolism and reduction of cytotoxic substances by PTX.