利用电穿孔技术将gfp表达质粒转染于SP2/0细胞,通过G418筛选获得稳定表达GFP的小鼠骨髓瘤细胞株SP2/0-GFP细胞。将稳定的转化细胞移植于同系BALB/c小鼠后腿、耳部皮下及腹腔成瘤,每隔一天对小鼠肿瘤成像,跟踪肿瘤的生长过程。结果表明,探测到的GFP荧光强度和发光范围与肿瘤的生长或消亡相关,从而证明可以利用GFP作为肿瘤细胞的标记,结合光学成像技术,对肿瘤生长过程实时追踪。 The gfp expression plasmid was transfected into SP2 / 0 cells by electroporation, and the mouse myeloma cell line SP2 / 0-GFP stably expressing GFP was screened by G418. The stable transformed cells were transplanted into the hind legs of the same line of BALB / c mice, subcutaneously and intraperitoneally into the ear. The mice were imaged every other day and the tumor growth was followed. The results showed that the detected GFP fluorescence intensity and luminescence range were correlated with the growth or disappearance of the tumor, which proves that GFP can be used as a marker of tumor cells. Combined with optical imaging technology, the GFP fluorescence can be used to track the tumor growth in real time.