GFP相关论文
被子植物的生长发育过程包括根的生长、叶的发育、花形态建成、减数分裂、双受精等一系列不断变化的事件,在这些过程中包含了一系......
猪繁殖与呼吸综合征（PRRS）,病原为猪繁殖与呼吸综合征病毒（PRRSV）,所有猪均易感。1987年,该病首次于美国发现,随后迅速席卷全球养猪业,......
学位
本案例依托人教版高中《生物学·选择性必修3·生物技术与工程》,通过整合教材,设计跨单元教学,组织学生开展了“转Gfp大肠杆菌作......
水是生命的重要要素,水的流入和流出细胞主要是通过细胞膜上存在的水孔蛋白完成的。植物水孔蛋白可分为四个家族,其中PIP1作为一种......
Na+驱动的细菌鞭毛马达，是由钠离子横跨细胞膜而引起的电化学势能为能量驱动的。鞭毛马达包括转子和定子两部分，它们都是由若干蛋白......
小麦（Triticum aestivum L.）,隶属禾本科（Gramineae）,小麦族,小麦属（Triticum L.）,是具有广泛适应性的重要粮食作物。受到全球气候变化、......
[目的]表面活性剂与0.25%胰酶共同作用制备脱细胞动脉基质。将通过GFP+慢病毒标记猪的胆管上皮细胞与脱细胞动脉基质共同培养后使......
无义介导的m RNA衰减（nonsense-mediated m RNA decay,NMD）是一种细胞内重要的RNA质量控制机制,可以确保含有提前终止密码子（prematur......
[目的]炭疽菌属(Colletotrichum spp.)病原菌主要引起茶树叶部炭疽病害.为了降低茶树受到炭疽菌的侵害,从而防控茶树炭疽病,作者进......
以本氏烟草（Nicotiana benthamiana）为植物材料,分析了不同农杆菌菌株（LBA4404菌株、EHA105菌株、GV3101菌株）、菌液浓度以及侵染时间......
小麦根腐离蠕孢[Bipolaris sorokiniana(Sacc.) Shoemaker]是一种能侵染小麦、大麦、燕麦等谷类作物的土传真菌.除了侵染作物的根......
Glyphosate inhibits the translocation of macromolecules in the parasitic association between Egyptia
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The whole network is changing to the data-centric network.How to carry data services over transport network has gone thr......
The Infection Process by a Green Fluorescent Protein-Tagged of Verticillium dahliae in Arabidopsis t
As a soil-borne phytopathogen, Verticillium dahliae Kleb (V.dahliae) causes verticillium wilt diseases by infection thro......
Biotrophy Lifestyle is Revealed in The Early Stage of Infection Process by GFP Labeled Strain of Bot
Botryosphaeria dothidea is a destructive pathogen of apple and pear.To investigate the infection process,binary vectors ......
随着IP和光网络技术的不断发展,IP与SDH技术的结合将越来越紧密.本文简要说明几种基于SDH的IP封装技术,对应用较为广泛的三种封装......
生防菌定殖的追踪和监测对于追踪微生物与宿主植物之间关系的研究是至关重要的。诸多试验表明,沼泽红假单胞菌能够明显促进植物生......
本研究选择了9株分离自菜豆(Phaselous vulgaris)和杭子梢(Campylotropis sp.)与悬钩子土壤杆菌同种的根瘤菌,对它们进行了gfp(绿......
目的 构建可被光学成像设备检测的人乳腺癌细胞株.方法 通过慢病毒感染整合表达的方式使人乳腺癌细胞MCF7细胞表达绿色荧光蛋白(GF......
Eicosapentenoic acid attenuates allograft rejection in an HLA-B27/EGFP transgenic rat cardiac transp
Objective: The development of an animal model bearing definite antigens is important to facilitate the evaluation and mo......
Eicosapentenoic acid attenuates allograft rejection in an HLA-B27/EGFP transgenic rat cardiac transp
Objective: The development of an animal model bearing definite antigens is important to facilitate the evaluation and mo......
Purpose The electroporation of mouse retinal ganglion cells (RGCs) is not efficient after birth,as transfection of the d......
The Study of Protein-protein interactions and energy transfer between photo protein clytin and GFP b
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Construction of eukaryotic expression plasmid about human retina-derived NT-3 and its expression, se
The human neurotrophin-3(NT-3) DNA was obtained by RT-PCR from retinal tissue.Expression plasmid of NT-3, p EGFP-N1-NT-3......
灵芝(Ganodermalucidum)是我国传统药物中有多种治疗和保健作用的药用真菌.本课题组于2012年6月发表了灵芝基因组的研究结果，推动灵......
A reliable and high-efficiency Agrobacterium tumefaciens-mediated transformation system of Pogonathe
Pogonatherum paniceum(Lam.)Hack,a perennial turfgrass,is important in ecological restoration and landscape construction ......
为了比较和探索SB,PB和Tol2三种转座子介导下,斑马鱼上的转基因效率和基因捕获效率研究,以及基因捕获个体的F1代的基因捕获效率；本......
蛋白质是模型的精确结构大分子，其序列、长度、立体化学甚至折叠的三维结构都是精确确定的。但是，天然蛋白质的主链结构多半是线性结......
Determination of scFv fragments number displayed on the surface of m13 phage by capillary electropho
Phage display technology is a powerful tool to find ligands with high affinities to given targets.Arguably the most succ......
Stabilization of Functional Reconstitution of Staphylococcus aureus AgrC Histidine Kinase into Artif
Elucidation of the molecular mechanisms of two-component signal transduction (TCST) system continues to be the most chal......
Generation of A Stable GFP-reporter Zika Virus System for High-throughput Screening of Zika Virus In
Zika virus(ZIKV) is associated with severe birth defects and Guillain-Barre′ syndrome and no approved vaccines or speci......
我们通过大规模的全基因组基因突变与筛选心脏发育候选基因，发现一个在斑马鱼心脏特定细胞表达的基因TSMR1.我们构建了TSMR1-GF......
会议
本文分析了EOS系统中常用的封装协议,讨论了多业务传送平台(MSTP)中采用的虚级联技术以及LCAS(链路容量调整方案),并简要介绍了EOS......
The Effects of a Short Sequence Enhancer (5-GTGAAATAAATGCAAATAAAGT)and its Derived Sequences on Gree
Objective To determine enhancer activity of a short sequence DNA (AA, 5-GTGAAATAAATGCAAATAAAGT) and its derived sequ......
Suitable Sequences and a Stem-Loop Structure are Required for cis Element-Activating Gene Expression
We have previously demonstrated that SV40PolyA in antisense orientation (PolyAas,240 bp) eliminated the inhibition of GF......
农杆菌介导的瞬时基因表达因其操作简单、可重复性高和实验成本低而成为研究基因功能、生产活性蛋白的有效方法。该研究以非病毒型......
期刊
目的构建含B7-1基因与绿色荧光蛋白(GFP)基因的融合表达载体,并转染LM8骨肉瘤细胞,研究获得其在LM8细胞中高效稳定表达的方法。方......
绿色荧光蛋白（green fluorescent protein, GFP）是一种在当今生命科学和医学研究中被广泛使用的示踪物。它的出现彻底改变了科研人员......
GATA-2, a transcription factor, is expressed in several types of blood cells and in the central nervous system (CNS), an......
Actins widely exist in eukaryotic cells and playimportant roles in many living activities. As there are manykinds of act......
GFP,即绿色荧光蛋白,是当代生物科学研究领域最重要的工具之一。它具有惊人的科学研究价值,在它的帮助下,研究人员可以直接看到生......
[目的]确定OsWRKY78蛋白在植物中的定位。[方法]根据GenBank数据库中OsWRKY78全序列设计引物,进行OsWRKY78的RT-PCR扩增,克隆了OsW......
GFP法依据模糊数学的理论,基于用计算机求解采矿方法评选课题,引入了归一化模糊正整数定义归类基准,建立了可编程的Fuzzy数学模型,......
目的：采用活细胞成像技术观察破骨细胞微管正端蛋白EB1在细胞内的分布及运动，初步研究微管在破骨细胞功能中的作用。方法①用脂质体......
目的:以绿色荧光蛋白(GFP)作为报道基因,探讨转染GFP的培养细胞检测细胞周期的流式细胞技术.方法:用贴壁细胞MCF-7细胞以脂质体转......
近年来,自体脂肪移植已被广泛应用于疤痕去除,烧伤后修复以及全身多处的整容填充修复重建[1,2]。与其他填充材料相比,自体脂肪移植......
