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目的:探讨体外循环低温室颤对犬肺功能的影响.方法:健康成年杂种犬14只随机分为实验组和对照组,每组7只.全麻,右侧第四肋间进胸,常规建立体外循环(CPB),心尖部左心引流.实验组:不阻断升主动脉,血流降温到28℃,心包腔内注入4℃生理盐水诱导室颤;对照组:阻断升主动脉,经主动脉根部灌注停跳液.检测不同时间点血清IL-8浓度、肺顺应性,观察肺组织形态学改变,比较体外循环条件下低温室颤对肺脏的损伤程度.结果:实验组与对照组的血清IL-8的浓度水平比较差异有统计学意义(P<0.05),各时间点之间也存在差异(P<0.05).两组在T1时间点的IL-8浓度无明显差别,转机后均开始升高,且均在T3时间点达到高峰,并于T4时间点逐渐下降,但仍然高于T1时间点水平.肺组织形态学显示:实验组较对照组肺组织病理损伤减轻;肺顺应性:实验组与对照组肺顺应性比较差异有统计学意义(P<0.05),各时间点之间也存在差异(P<0.05).两组在T1时间点肺顺应性无明显差别,转机后均开始降低,且均在T3时间点达到高峰,并于T4时间点逐渐恢复,但仍然低于T1时间点水平.结论:体外循环中应用低温室颤技术可降低组织的损伤程度,对肺功能有保护作用.
Objective: To investigate the effects of hypothermia VFD on lung function of dogs during cardiopulmonary bypass.Methods: Fourteen healthy adult dogs were randomly divided into experimental group and control group, with 7 in each group.Anesthesia, right intercostal intercostal space, routine The establishment of cardiopulmonary bypass (CPB), apical left ventricular drainage.Experimental group: without blocking the ascending aorta, blood flow cooling to 28 ℃, pericardial injection of 4 ℃ saline induced ventricular fibrillation; control group: blocking the ascending aorta , The aorta was perfused with cardioplegic solution at different time points.Serum IL-8 concentration and lung compliance were measured at different time points to observe the morphological changes of the lung tissue and to compare the degree of lung damage induced by low temperature ventricular fibrillation under cardiopulmonary bypass.Results: The level of IL-8 in serum of the control group was significantly lower than that of the control group (P <0.05), there were also differences between the time points (P <0.05) , Both of them began to increase after turning point, and all reached the peak at T3, and gradually decreased at T4, but still higher than the T1 time point.The histopathology of lung showed that the pathological damage of lung in experimental group was lighter than that in control group ; Lung compliance: There was significant difference in lung compliance between experimental group and control group (P <0.05) (P <0.05) .There was no significant difference in lung compliance at T1 time point between the two groups, and all of them decreased after turning point, and all reached the peak at T3 time point and recovered gradually at T4 time point , But still lower than the T1 time point.Conclusion: Applying hypothermia ventricular fibrillation during cardiopulmonary bypass can reduce the degree of tissue damage and protect lung function.