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[Objective] The aim was to explore an optimized method to measure the artificial pigment by means of high performance liquid chromatograph.[Method] The gradient elution technique of HPLC was used to isolate and analyze tartrazine and sunset yellow in the cereals.The pre-treatment temperature,ultrasonic time,the column temperature and the elution conditions were studied,and the best experimental conditions were confirmed.[Result] At 40 ℃,the optimum chromatography analysis condition was moving phase,with pH(6.8),methanol/water of acetic acid-ammonium acetate(0.02 mol/L),and column temperature(35 ℃).Gradient elution condition was 20%-35% methanol,3%/min;35%-98%,6%/min;98%,8 min.The content of tartrazine and sunset yellow in cereal was tested precisely by the means of HPLC.The average recoveries of standard addition were 97.9% and 100.2%,and the detection limit were 0.02 mg/kg and 0.03 mg/kg.[Conclusion] This method shortened the analysis time,improved the detection sensitivity and the resolution of artificial color.
[Objective] The aim was to explore an optimized method to measure the artificial pigment by means of high performance liquid chromatograph. [Method] The gradient elution technique of HPLC was used to isolate and analyze tartrazine and sunset yellow in the cereals. The pre- Treatment temperature, ultrasonic time, the column temperature and the elution conditions were studied, and the best experimental conditions were confirmed. [Result] At 40 ° C, the optimum chromatography analysis condition was moved phase, with pH (6.8), methanol / water of acetic acid-ammonium acetate (0.02 mol / L), and column temperature (35 ° C). Gradient elution condition was 20% -35% methanol, 3% / min; 35% -98%, 6% / min; 8 min. The content of tartrazine and sunset yellow in cereal was tested precisely by the means of HPLC. The average recoveries of standard addition were 97.9% and 100.2%, and the detection limit were 0.02 mg / kg and 0.03 mg / kg. Conclusion] This method shortened the analysis time, improved the detection sensitivity and th e resolution of artificial color.