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目的:探讨苦参碱对腹膜透析大鼠腹膜纤维化的影响和可能的机制。方法:采用高糖透析液+脂多糖复制腹膜纤维化模型。将大鼠随机分为对照组、模型组、苦参碱低剂量组和苦参碱高剂量组。治疗组在造模同时每日腹腔内注射不同剂量的苦参碱。35d后处死各组大鼠,行2h腹膜平衡试验,测量超滤量,检测腹膜功能;留取腹膜组织行病理切片做HE及Masson染色观察腹膜组织学改变;用RT-PCR法测定腹膜血管内皮生长因子(VEGF)和纤维连接蛋白(FN)表达率。结果:苦参碱治疗组的腹膜厚度和血管增生程度比模型组明显减轻,VEGF和FN的合成水平也明显下降。结论:苦参碱可以有效的防治腹膜纤维化的进程。
Objective: To investigate the effect and possible mechanism of matrine on peritoneal fibrosis in peritoneal dialysis rats. Methods: High glucose dialysate + lipopolysaccharide replicate peritoneal fibrosis model. The rats were randomly divided into a control group, a model group, a low-dose matrine group, and a high-dose matrine group. In the treatment group, different doses of matrine were injected intraperitoneally at the same time. After 35 days, each group of rats was sacrificed and peritoneal balance test was performed for 2 hours. The ultrafiltration volume was measured and the peritoneal function was measured. Peritoneal tissue was taken and pathological sections were performed for HE and Masson staining to observe the histological changes of the peritoneum; RT-PCR method was used to measure peritoneal vascular endothelium. Growth factor (VEGF) and fibronectin (FN) expression rates. RESULTS: Compared with the model group, the peritoneal thickness and vascular hyperplasia of the matrine-treated group were significantly reduced, and the synthesis of VEGF and FN was also significantly decreased. Conclusion: Matrine can effectively prevent and treat peritoneal fibrosis.