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试管组织分离常常由于细菌、酵母菌污染导致失败,既耽误了时间又浪费了培养基,实是遗憾。现将此法操作略作改进,可达到分离成功的目的。(一)试管组织分离培养法:子实体选取、消毒、培养基制作均同常规,只是在接种时,使试管培养基的斜面朝下,将组织块放于试管棉塞前的内壁上,塞好棉塞后竖立试管让组织块沿壁下滑达管的底部并接触培养基。注意组织块下滑时不能接触到培养基,这样即使组织块带菌也不至于污染整个培养基斜面。接种后将试管立放或斜放,斜放程度不得低于培养基在
Test tube tissue separation often due to bacteria, yeast contamination led to failure, not only wasted time and waste of media, it is regrettable. This method is now slightly improved operation, can achieve the purpose of separation success. (A) test tube tissue culture method: fruiting body selection, disinfection, medium production are the same as conventional, but inoculation, the test tube medium slant down, the tissue block placed on the inner wall of the test tube before the tampon, plug After a good tampon, erect the test tube and allow the tissue block to slide down the bottom of the tube and contact the medium. Note that there is no access to the culture media when the tissue slides down, so that the entire media ramp is not contaminated even if the tissue mass is carried. After inoculation will test tube standing or diagonal, oblique degree of not less than medium in