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研究壳寡糖对大肠杆菌的抑菌作用,其中大肠杆菌胞外碱性磷酸酶(AKP)活性的变化用来评价壳寡糖对菌体通透性的影响,通过测定活性氧相对含量、丙二醛(MDA)含量、蛋白质羰基化和DNA氧化损伤,评价壳寡糖对大肠杆菌的氧化损伤作用。结果表明,0.5%和1.0%壳寡糖对大肠杆菌的抑制效果显著,同时破坏了大肠杆菌的菌体完整性。大肠杆菌在壳寡糖的处理下,随着时间推移活性氧的相对含量逐渐降低,膜质的氧化损伤及蛋白质的氧化损伤严重;在0.5%和1.0%壳寡糖的处理下,DNA氧化损伤作用不明显。诱导活性氧的产生,引起膜质和蛋白质的氧化损伤,在其抑菌过程中发挥重要作用。
To study the antibacterial activity of chitooligosaccharides against Escherichia coli, the changes of Escherichia coli extracellular alkaline phosphatase (AKP) activity were used to evaluate the effect of chitooligosaccharides on the permeability of Escherichia coli. By measuring the relative content of reactive oxygen species, Dialdehyde (MDA) content, protein carbonylation and DNA oxidative damage, to evaluate the effect of chitooligosaccharides on the oxidative damage of Escherichia coli. The results showed that 0.5% and 1.0% chitooligosaccharides significantly inhibited Escherichia coli, at the same time undermining the E. coli bacterial cell integrity. Under the treatment of chitosan oligosaccharide, the relative content of reactive oxygen species gradually decreased, the oxidative damage of membrane and the oxidative damage of protein were serious. With the treatment of 0.5% and 1.0% chitooligosaccharides, DNA oxidative damage The role is not obvious. Induction of reactive oxygen species, causing membrane and protein oxidative damage, play an important role in its antimicrobial process.