目的 :探讨端粒酶活性与人乳腺良、恶性病变组织之间的关系。方法 :用 TRAP- EL ISA检测法 ,对 15例乳腺良性病变和 7例乳腺癌组织进行端粒酶活性水平检测。结果 :7例乳腺浸润性导管癌中 ,5例端粒酶阳性 ,而 15例乳腺良性疾病中仅 2例端粒酶阳性 ,两组差异有显著性 (P<0 .0 5 )。此外 ,有淋巴结转移与无淋巴结转移的乳腺癌组织中端粒酶活性水平差异无显著性 (P>0 .0 5 )。结论 :端粒酶的激活在人类乳腺癌的发生过程中可能起重要作用 ,且端粒酶有可能成为鉴别乳腺良恶性病变和诊断乳腺癌的肿瘤标记物 Objective: To investigate the relationship between telomerase activity and benign and malignant lesions in human breast. Methods: Telomerase activity was detected in 15 benign breast lesions and 7 breast cancer tissues by TRAP-ELISA. Results: Of the 7 cases of invasive ductal carcinoma of the breast, 5 were positive for telomerase, while only 2 of 15 cases of benign breast disease were positive for telomerase. There was significant difference between the two groups (P <0.05). In addition, there was no significant difference in telomerase activity between breast cancer with lymph node metastasis and non-lymph node metastasis (P> 0.05). Conclusion: Activation of telomerase may play an important role in the development of human breast cancer, and telomerase may become a tumor marker for differential diagnosis of benign and malignant breast lesions and breast cancer