花药离体培养工作中,接种花粉的发育时期,对诱导花粉产生愈伤组织或胚状体的频率是个很敏感的因素,这实质是离体培养的花粉生理适宜度问题,因而也是花药培养的重要条件。产生最高诱导频率的花粉发育时期称为花粉的发育适期。具体掌握花粉适期问题,对于提高接种花粉的准确性,提高诱导频率都有着重要的实践意义,也是研究各项花培技术的实验前提。一般实践中提高 In vitro culture of anthers, the period of inoculation of pollen is a very sensitive factor in inducing the frequency of callus or embryoid body formation in pollen, which is essentially the question of the physiological suitability of pollen in vitro and therefore also the anther culture Important condition. The period of pollen development that produces the highest induction frequency is called the pollen developmental period. Specific control of pollen maturity issues, to improve the accuracy of vaccination pollen, to improve the induction frequency has an important practical significance, but also to study the various techniques of flower culture experimental premise. Increase in general practice