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目的初步探索青海少数民族地区输血后肝炎病毒TTV感染的分子流行病学和TTVDNA部分核苷酸片段序列的分析。方法采用ELISA聚合酶链反应PCR技术,检测藏族、蒙古族、土族人群TTV的感染性,TTVDNA应用荧光法测序分析部分核苷酸片段。结果ELISA检测74例藏族TTV阳性率14.9%;57例蒙古族TTV阳性率1.9%;土族未检测到阳性。PCR检测13例TTV阳性血清,DNA阳性率53.8%;藏族TTVDNA部分核苷酸测序,对照BDH1.Shanxi13.Gla序列,同源性达90%以上。结论TTV在青海少数民族人群间有一定的感染性,不同区域的民族感染率有显著差异。藏族株TTVDNA片段对照分析同源性为90%以上,属Gla亚型。提示民族间感染有输入性特征。
Objective To explore the molecular epidemiology and partial nucleotide sequence of TTVDNA in post-transfusion hepatitis B virus infection in Qinghai minority areas. Methods TTV infection in Tibetan, Mongolian and Tu ethnic groups was detected by ELISA, and some nucleotide fragments were analyzed by TTVDNA. Results The positive rate of TTV in 74 Tibetans was 14.9% by ELISA. The positive rate of TTV in 57 Mongolians was 1.9%. No positive result was found in Tu people. 13 TTV positive sera were detected by PCR and the positive rate of DNA was 53.8%. The nucleotide sequence of TTVDNA of Tibetans was compared with the sequence of BDH1.Shanxi13.Gla and the homology was over 90%. Conclusion TTV has some infectivity among ethnic minority population in Qinghai Province, and there are significant differences in ethnic infection rates in different regions. The TTTVNA fragment of Tibetan strain has a homology of more than 90%, belonging to the Gla subtype. Prompted inter-ethnic infection with input characteristics.