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目的观察磁刺激对离体新生大鼠神经干细胞生长和分化的影响。方法利用无血清培养技术,从新生大鼠脑室下区分离培养神经干细胞。将神经干细胞置于0.5Hz,刺激强度分别为0.48T(25%最大输出,B组)、0.95T(50%最大输出,C组)和1.44T(75%最大输出,D组)的条件下进行磁刺激干预,每天1次,每次30个脉冲,作用3d,同时设立对照组(A组),用四甲基偶氮唑蓝(MTT)法检测细胞活性(OD值),并用流式细胞技术检测神经干细胞分化情况。结果磁刺激各组神经干细胞在干预后24~48hOD值较A组稍低(P<0.05),至72h后可恢复至A组水平;流式结果显示磁刺激各组神经丝蛋白(NF)阳性细胞比例均高于A组,其中D组NF阳性神经元的比例最高(P<0.05)。结论0.5Hz磁刺激对神经干细胞增殖有一过性的轻度抑制作用,有利于神经干细胞向神经元方向的分化。
Objective To observe the effects of magnetic stimulation on the growth and differentiation of neural stem cells in isolated neonatal rats. Methods Neural stem cells were isolated and cultured from the subventricular zone of neonatal rat using serum-free culture. The neural stem cells were exposed to 0.5 Hz with stimulation intensities of 0.48T (25% maximal output, group B), 0.95T (maximal 50% output, group C) and 1.44T maximal output (group D, 75% The rats were randomly divided into three groups: control group (group A), MTT assay (MTT), cell viability (OD), flow cytometry Cell technology to detect neural stem cell differentiation. Results After stimulated by magnetic stimulation, the value of OD of neural stem cells of each group was slightly lower than that of A group (P <0.05) 24 h after intervention, and returned to the level of A after 72 h. The results of flow cytometry showed that the neurofilament (NF) The percentage of NF-positive neurons in group D was the highest (P <0.05). Conclusion 0.5 Hz magnetic stimulation has a transient mild inhibitory effect on the proliferation of neural stem cells, which is conducive to the differentiation of neural stem cells to neurons.