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目的:探讨程序性细胞死亡因子4(PDCD4)与喉鳞状细胞癌发生和发展的关系。方法:免疫组织化学方法和Western印迹法检测喉鳞状细胞癌组织及癌旁正常喉黏膜组织中PDCD4蛋白表达。结果:免疫组织化学染色显示喉鳞状细胞癌组织中PDCD4蛋白阳性表达率低于癌旁正常喉黏膜组织,差异有统计学意义(P<0.01)。喉鳞状细胞癌组织中PDCD4蛋白阳性表达和患者的年龄、性别、临床分型(肿瘤部位)、TNM分期无相关性,与病理分级、淋巴结转移有显著相关性。免疫印迹结果亦显示喉鳞状细胞癌组织中PDCD4蛋白表达低于癌旁正常喉黏膜组织(P<0.01)。结论:PDCD4蛋白低表达与喉鳞状细胞癌的发生和发展有关,PDCD4可能成为预测喉鳞状细胞癌转移和预后的生物学指标。
Objective: To investigate the relationship between the expression of programmed cell death factor 4 (PDCD4) and the occurrence and development of laryngeal squamous cell carcinoma. Methods: Immunohistochemistry and Western blotting were used to detect the expression of PDCD4 protein in laryngeal squamous cell carcinoma and normal laryngeal mucosa. Results: Immunohistochemical staining showed that the positive rate of PDCD4 protein in laryngeal squamous cell carcinoma was lower than that in normal laryngeal squamous cell carcinoma, the difference was statistically significant (P <0.01). The positive expression of PDCD4 in laryngeal squamous cell carcinoma was not correlated with the age, sex, clinical type (tumor location) and TNM stage, but also with the pathological grade and lymph node metastasis. Western blot also showed that the expression of PDCD4 protein in laryngeal squamous cell carcinoma was lower than that in normal laryngeal mucosa (P <0.01). Conclusion: The low expression of PDCD4 protein is associated with the occurrence and development of laryngeal squamous cell carcinoma. PDCD4 may be the biological index for predicting the metastasis and prognosis of laryngeal squamous cell carcinoma.