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目的 研究参麦注射液对化学性肝脏损伤的保护作用。方法 将 90只成年雄性健康SD大鼠随机分成正常对照组、四氯化碳 (carbontetrachloride ,CTC)组、参麦 (Shenmai)保护组。后两组皮下注射 50 %CTC油 3ml/kg ,每周 2次 ,共 12周 ,Shenmai保护组每天给予参麦注射液 1ml/kg灌胃。于第 4周、第 8周、第12周 ,每组各处死 8只大鼠 ,取肝组织测定脂质过氧化物代谢产物丙二醛 (MDA)及超氧化物歧化酶 (SOD) ,取下腔静脉血测定血清谷丙转氨酶 (ALT)、谷草转氨酶 (AST)、乳酸脱氢酶 (LDH)、一氧化氮 (NO)。结果 Shenmai保护组肝组织SOD含量明显高于CTC组 (P <0 .0 1) ,MDA含量明显低于CTC组 (P <0 .0 1) ;血清ALT、AST、LDH含量明显低于CTC组 (P <0 .0 1) ;NO含量明显高于CTC组 (P <0 .0 1)。结论 参麦注射液对CTC所致的肝脏损伤有明显的保护作用 ,其机理可能与提高肝细胞耐缺氧能力 ,清除氧自由基 ,影响NO含量等有关
Objective To study the protective effect of Shenmai injection on chemical liver injury. Methods Ninety adult male Sprague-Dawley rats were randomly divided into normal control group, carbon tetrachloride (CTC) group and Shenmai protection group. After the two groups were injected subcutaneously with 5 % CTC oil 3 ml/kg twice a week for 12 weeks. The Shenmai protection group was given 1 ml/kg of Shenmai injection daily. At the 4th, 8th, and 12th week, 8 rats in each group died. The liver tissues were assayed for lipid peroxide metabolites malondialdehyde (MDA) and superoxide dismutase (SOD). Serum ALT, AST, LDH, and NO were measured in inferior vena cava. Results The content of SOD in liver tissue of Shenmai group was significantly higher than that of CTC group (P<0.01), and the content of MDA was significantly lower than that of CTC group (P<0.01). The levels of serum ALT, AST and LDH were significantly lower than those of CTC group. (P <0. 0 1); NO content was significantly higher than that of CTC group (P <0. 01). Conclusion Shenmai injection has obvious protective effect on liver injury caused by CTC. The mechanism may be related to the improvement of hepatocyte resistance to hypoxia, clearance of oxygen free radicals, and affecting NO content.