在盐酸介质中,基于痕量头孢氨苄(CPX)对β-环糊精(β-CD)增敏KBrO3-KBr氧化亚甲蓝(MB)体系的荧光具有增强作用,提出了一种新的测定头孢氨苄荧光分析方法。该体系最大激发波长为λex=660 nm,最大发射波长为λem=678 nm,线性范围为0.001～3.5 mg/L,检出限为0.0002 mg/L,相对标准偏差为1.3%。方法可作为痕量头孢氨苄的分析方法。 In hydrochloric acid medium, a new assay based on trace amount of cefalexin (CPX) for enhancing the fluorescence of KBrO3-KBr oxidized methylene blue (MB) system stimulated by [beta] -cyclodextrin Cephalosporin fluorescence analysis method. The maximum excitation wavelength λex = 660 nm, the maximum emission wavelength λem = 678 nm, the linear range of 0.001 ~ 3.5 mg / L, the detection limit of 0.0002 mg / L, the relative standard deviation of 1.3%. Method can be used as a trace of cephalexin analysis.