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目的优化河豚鱼皮胶原寡肽的制备工艺。方法以河豚鱼皮为原料,制备河豚鱼皮胶原寡肽螯合锌,以含锌量为实验指标,研究了pH值、温度、时间、胶原寡肽与锌的摩尔比对螯合反应的影响,在单因素实验的基础上采用4因素3水平的Box-Behnken响应面分析法,对螯合工艺进行优化。结果在pH 5.3,反应温度70.1℃,反应时间2.9h,胶原寡肽与硫酸锌的摩尔比2.1∶1的优化条件下,制备的胶原寡肽螯合锌的含锌量最高,达到14.18%。红外光谱分析表明,螯合后胶原肽中的C=O,N-H都参与了Zn2+的配位,生成了胶原寡肽螯合锌。结论响应面法能很好地应用于河豚鱼皮胶原寡肽螯合锌螯合工艺的优化。
Objective To optimize the preparation process of puffer fish collagen collagen oligopeptide. Methods The puffer fish skin was used as raw material to prepare chelated zinc oligopeptide chelated zinc. The effects of pH value, temperature, time, molar ratio of collagen oligopeptide to zinc on the chelating reaction were studied Based on the single factor experiment, Box-Behnken response surface analysis with 4 factors and 3 levels was used to optimize the chelation process. Results Under the optimal conditions of pH 5.3, reaction temperature 70.1 ℃, reaction time 2.9h and the molar ratio of collagen oligopeptide to zinc sulfate 2.1:1, the zinc content of collagen oligopeptide chelated Zn was the highest, reaching 14.18%. Infrared spectrum analysis showed that C = O and N-H in the chelated collagen peptide are all involved in the coordination of Zn2 +, resulting in collagen oligopeptide chelated zinc. Conclusion Response surface methodology can be applied to optimize chelation of chelated zinc oligopeptide by puffer fish collagen.