关于大肠癌的染色体研究,国外文献中虽有一些报道,但往往只用恶性渗出液或癌组织的直接法进行研究,忽略培养条件在核型选择中的重要作用,对建株细胞的染色体研究更缺乏报道。我们对一株大肠癌细胞(Vaco 5)在培养中用不同的方法处理,结果得到不一致的核型,说明培养条件的改变可对核型产生一定的影响。 Although studies on chromosomes of colorectal cancer have been reported in foreign literature, only direct methods of malignant exudate or cancer tissue are often used to study, ignoring the important role of culture conditions in the selection of karyotypes, and the chromosomes of established cell lines. Research is even less reported. We treated a colorectal cancer cell (Vaco 5) with different methods in culture, resulting in inconsistent karyotypes, indicating that changes in the culture conditions can have a certain impact on the karyotype.