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基于生化分子检测技术高灵敏度、小型化的需求,近些年国内外相继提出一种用光纤表面增强拉曼散射(SERS)探针进行拉曼信号检测的方法,此检测方法不仅能实现远端检测和原位检测功能,而且具有很高的灵敏度.本文通过简单的管式腐蚀法制成一种锥柱组合型光纤探针,并通过静电引力将银纳米颗粒结合到硅烷化的二氧化硅光纤探针表面.用罗丹明6G(R6G)溶液的检测极限来表征该光纤探针的活性和灵敏度,通过优化银纳米颗粒的自组装时间为30 min,光纤探针直径为62μm,制备出高灵敏度的光纤SERS探针,远端检测R6G的检测极限可达到10~(-14)mol/L.因此,该光纤SERS探针在分子检测方面有巨大的应用前景.
Based on the demand of high sensitivity and miniaturization of biochemical molecular detection technology, in recent years, a method of Raman signal detection using optical fiber surface enhanced Raman scattering (SERS) probes has been proposed one after another, and this detection method can not only achieve remote Detection and in-situ detection, but also has high sensitivity.In this paper, a cone-column combination fiber probe was fabricated by a simple tube-like etching method and silver nanoparticles were electrostatically attracted to the silanated silica fiber probe The surface activity of the probe was characterized by the detection limit of rhodamine 6G (R6G) solution. By optimizing the self-assembly time of the silver nanoparticle for 30 min and the diameter of the fiber probe to be 62 μm, a highly sensitive Optical fiber SERS probe, the detection limit of distal detection R6G can reach 10 ~ (-14) mol / L. Therefore, the fiber SERS probe has great application prospect in molecular detection.