论文部分内容阅读
Spiroplasma has been successfully isolated from the honey bee (Apis mellifera) and cultivated in R-2 medium in China. The isolate, CH-1 is obtained through triple cloning. CH-1 shows a helical morphology and a contractile movement. It is 0.17 μm in diameter and its length varies considerably with different growth periods. CH-1 forms granular-type and/or fried-egg-shaped colonies, 75—210 μm in diameter, on R-2 medium. It grows normally at temperatures ranging 25—35℃ (the optimum being 28—32℃). The optimal pH for growth is 7.0. The best growth occurs in medium containing 0.25 mol/L of sucrose. Under the optimal condition, its growth reaches the stationary phase 48 h after subculture. CH-1 cannot grow in serum-free medium and its growth is inhibited by digitonin. CH-1 can metabolize glucose, fructose, maltose, and trehalose. It can dehydrate arginine, CH-1 does not have an ability to utilize urea, nor dehydrate gelatin, nor reduce methylence blue. The taxonomic position of CH-1 has also been discuss
Spiroplasma has been successfully isolated from the honey bee (Apis mellifera) and cultivated in R-2 medium in China. The isolate, CH-1 obtained through triple cloning. CH-1 shows a helical morphology and a contractile movement. It is 0.17 μm in diameter and its length varies considerably with different growth periods. CH-1 forms granular-type and / or fried-egg-shaped colonies, 75-210 μm in diameter, on R-2 medium. -35 ° C (the optimum being 28-32 ° C). The optimal pH for growth is 7.0. The best growth in medium containing 0.25 mol / L of sucrose. Under the optimal condition, its growth reaches the stationary phase for 48 h after subculture CH-1 can not grow in serum-free medium and its growth is inhibited by digitonin. CH-1 can metabolize glucose, fructose, maltose, and trehalose. It can dehydrate arginine, CH-1 does not have an ability to utilize urea, nor dehydrate gelatin, nor reduce methylence blue. The taxonomic position of CH- 1 has also been discuss