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为明确江苏省南京地区植株叶片表现斑驳症状的辣椒病毒病病原,针对性地开展该病的防治工作提供理论依据。2014年,我们对江苏省南京地区辣椒病毒病发生情况进行了调查,采集了70份表现斑驳症状的叶片样品,通过提取样品总RNA,分别用烟草花叶病毒属的简并引物Tob-Uni1/Tob-Uni2和辣椒轻斑驳病毒的特异性引物PMMo V-F/PMMo V-R进行RT-PCR分子检测,经序列测定、BLAST分析和MEGA6系统进化分析对病原进行鉴定。RT-PCR结果显示,在70份样品中,61份样本扩增到804 bp(烟草花叶病毒属病毒)和576 bp(辣椒轻斑驳病毒)的预期目的片段,检出率高达87.14%;序列BLAST分析结果显示,样品病毒与辣椒轻斑驳病毒的同源性最高(>98.3%),暗示其为PMMo V;MEGA6系统进化分析表明南京的辣椒病毒病病原属于PMMo V。2014年江苏省南京地区植株叶片表现斑驳症状的辣椒病毒病病原为烟草花叶病毒属的辣椒轻斑驳病毒,该病毒近年来在江苏首次发现。
In order to provide a theoretical basis for the prevention and control of pepper disease virus in Nanjing, Jiangsu Province, which has the mottled symptom of plant leaves, In 2014, we investigated the occurrence of pepper virus disease in Nanjing, Jiangsu province, and collected 70 samples of mottle-resistant leaves. By extracting the total RNA of the samples, we used Tob-Uni1 / Tob-Uni2 and PMMo VF / PMMo VR were detected by RT-PCR. The pathogen was identified by sequence analysis, BLAST analysis and MEGA6 phylogenetic analysis. RT-PCR results showed that out of 70 samples, 61 samples were amplified to 804 bp (Tobacco mosaic virus) and 576 bp (capsicum mottle virus). The detection rate was as high as 87.14% BLAST analysis showed that the homology of the sample virus to the capsid mottle virus was the highest (> 98.3%), which indicated that it was PMMoV. The evolutionary analysis of MEGA6 system indicated that the pepper virus pathogen in Nanjing belonged to PMMoV. In 2014, the pathogen of pepper virus disease with plant leaf mottled in Nanjing, Jiangsu Province, was the chili light mottle virus of tobacco mosaic virus, which was first discovered in Jiangsu in recent years.