论文部分内容阅读
目的研究A(H1N1)甲型流感病毒基因多态性对病毒复制及毒力的影响。方法选择A/Californ ia/04/2009,A/S ichuan/1/2009和A/Be ijing/3/2009 3株病毒,分别感染MDCK细胞,测定病毒RNA载量和病毒效价;同时滴鼻接种BALB/c小鼠,检测小鼠感染后的多项指标,观察期为14天。另一方面,对3株病毒的全基因组进行测序及序列比对,对其基因突变情况及多态性进行分析。结果 A/S ichuan/1/2009株相较而言具有较高的复制能力及毒力,序列分析结果显示全基因中有5个氨基酸发生了突变,其中PA蛋白中343位点的A突变为T,PB1蛋白中353位点的K突变为R,566位点的T突变为A,PB2蛋白中471位点的T突变为M,对病毒毒力的增强起重要作用。结论 A(H1N1)甲型流感病毒基因组多态性与其复制及毒力密切相关。
Objective To study the effect of A (H1N1) influenza A virus gene polymorphism on virus replication and virulence. Methods MDCK cells were infected with three strains of A / Californiana / 04/2009, A / S Sichuan / 1/2009 and A / Beijing / 3/2009 respectively. The viral RNA load and virus titer were determined. Inoculation of BALB / c mice, detection of a number of indicators of mouse infection, the observation period of 14 days. On the other hand, we sequenced and sequenced the genomes of the three viruses and analyzed their gene mutations and polymorphisms. Results A / S / 1/2009 strain had higher replication and virulence compared with other strains. Sequence analysis revealed five amino acid mutations in the whole gene. The A mutation at position 343 in PA protein was The mutation of K at position 353 of T and PB1 was R, the mutation of T at 566 was A, and the mutation of T at 471 of PB2 was M, which played an important role in the enhancement of virus virulence. Conclusion The polymorphism of A (H1N1) influenza A virus is closely related to its replication and virulence.