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目的:建立女贞子中红景天苷的HPLC的含量测定方法,测定女贞子不同市售品、不同炮制品及不同果实部位中红景天苷含量,为女贞子的质量控制研究提供参考。方法:回流提取法获得供试品溶液,HPLC法测定其含量;色谱条件为Kromasil-C18,250 mm×4.6 mm,5μm;流动相:甲醇-水(17∶83);流速:1 mL.min-1;柱温:40℃;检测波长:275 nm。结果:采用此HPLC法检测药材中红景天苷在0.25~5.02μg范围内r=0.999 9,表明线性关系良好,回归方程为Y=2.90×105X+9.30×103;回收率为99.52%,RSD为2.95%。结论:该方法能够为女贞子的质量控制提供参考数据。
OBJECTIVE: To establish a HPLC method for the determination of salidroside in Fructus Ligustri Lucidi, to determine the content of salidroside in different commercial products, different processed products and different fruit parts of Ligustrum lucidum, and to provide the quality control study for Ligustrum lucidum reference. Methods: The sample solution was obtained by reflux extraction and the content was determined by HPLC. The chromatographic conditions were Kromasil-C18, 250 mm × 4.6 mm, 5 μm. The mobile phase was methanol-water (17:83). The flow rate was 1 mL.min -1; column temperature: 40 ℃; detection wavelength: 275 nm. Results: Using this HPLC method, the linear range of salidroside in the range of 0.25 ~ 5.02μg was 0.999 9, the regression equation was Y = 2.90 × 105X + 9.30 × 103, the recovery was 99.52%, RSD 2.95%. Conclusion: This method can provide reference data for the quality control of Ligustrum lucidum.