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[目的]观察不同浓度的氢溴酸槟榔碱(Ah)对结肠平滑肌细胞钙离子(Ca2+)移动的影响,以探讨其对平滑肌收缩力的作用。[方法]取培养大鼠结肠平滑肌细胞,以荧光指示剂Fluo-3/AM负载,通过激光共聚焦显微镜(LSCM)动态观察不同浓度Ah对培养的结肠平滑肌细胞Ca2+移动的作用。[结果]Ah在1×10-10、1×10-9、1×10-8、1×10-7、1×10-6、1×10-5、1×10-4mol/L浓度刺激下可使结肠平滑肌细胞[Ca2+]i荧光强度(FI)逐渐升高(P<0.05),而在未加药以及1×10-13、1×10-12、1×10-11mol/L浓度刺激下对[Ca2+]iFI无影响(P>0.05);在1×10-3mol/L浓度刺激下细胞膜迅速发生破裂;应用阿托品预处理,则FI从4.28±1.51降至3.83±1.40(P>0.05)。[结论]Ah可使结肠平滑肌细胞内[Ca2+]i逐渐升高,阿托品可阻断Ah对平滑肌的收缩作用。
[Objective] To observe the effect of different concentrations of arecoline hydrobromide (Ah) on the calcium ion (Ca2+) movement of colonic smooth muscle cells to explore its effect on the contractility of smooth muscle. [Method] Cultured rat colonic smooth muscle cells were loaded with fluorescent indicator Fluo-3/AM, and the effects of different concentrations of Ah on Ca2+ migration of cultured colonic smooth muscle cells were dynamically observed by laser confocal microscope (LSCM). [Results] Ah stimulated at concentrations of 1×10-10, 1×10-9, 1×10-8, 1×10-7, 1×10-6, 1×10-5, 1×10-4 mol/L Under normal conditions, the [Ca2+]i fluorescence intensity (FI) of colonic smooth muscle cells increased gradually (P<0.05), while no drug and 1×10-13, 1×10-12, and 1×10-11 mol/L concentrations were observed. There was no effect on [Ca2+]iFI under stimulation (P>0.05); the cell membrane ruptured rapidly under the stimulation of 1×10-3 mol/L; FI was decreased from 4.28±1.51 to 3.83±1.40 after atropine pretreatment. 0.05). [Conclusion] Ah can increase [Ca2+]i in colonic smooth muscle cells gradually. Atropine can block the contraction of Ah on smooth muscle.