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材料与方法 1.实验动物及喂药方法:见本实验研究(二)(本刊,1986,第1期,第39页)。2.体外淋巴细胞存活率观察:将每毫升含1×10~7 的脾细胞混悬于含有双抗、小牛血清之1640培养液中,置于37℃、CO_2培养箱中孵育,间隔5、24、48、72小时取出,用台盼蓝染色,计算200只淋巴细胞,算出其存活率。3.自身花环试验:取小鼠血0.5毫升肝素抗凝后加等量Hank氏液,充分混合,轻轻放置于淋巴细胞分离液(比重为1.090±0.001)上层,3500npm/分速度离心20分钟,分别吸取第二层之雾状的淋巴细
Materials and Methods 1. Experimental animals and feeding methods: See this experimental study (2) (Journal of 1986, No. 1, p. 39). 2. Observation of viability of lymphocytes in vitro: Spleen cells containing 1×10~7 cells per ml were suspended in 1640 culture medium containing double antibody and calf serum and incubated in a CO 2 incubator at 37°C. After 24, 48, and 72 hours, the cells were stained with trypan blue and 200 lymphocytes were counted to calculate the survival rate. 3. Self-garland test: take 0.5 ml of heparin from mouse blood and add Hank’s solution equal to anticoagulation. Mix well and gently place it on the upper layer of lymphocyte separation fluid (specific gravity: 1.090±0.001). Centrifuge at 3500npm/min for 20 minutes. , drawing the second layer of hazy lymphatic