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用根内球囊霉、摩西球囊霉、地表球囊霉及其混合菌剂接种无菌根枳橙幼苗进行盆栽试验,研究接种AM真菌对枳橙幼苗根际土壤微生态环境的影响,结果表明:接种AM真菌的根系形成了40%～70%的菌根侵染率;菌根枳橙的苗高、地茎、地上部/地下部生物量等营养生长显著增加;AM真菌对根际微生物种群数量产生一定的影响,使根区细菌、放线菌、真菌、固氮菌、氨化菌、纤维素分解菌的数量和微生物生物量显著增加(p<0.05);接种AM真菌增加了根际土壤碱性磷酸酶、脲酶的活性,各种酶活性与菌根侵染率呈极显著正相关(p<0.01);接种菌根的根际土壤中,可直接被植物吸收利用的N、P元素出现富集现象,且N、P含量与菌根侵染率呈极显著正相关.通径分析结果表明:枳橙根际土壤微生物、酶活性、pH及养分特性通过直接和间接作用共同影响着菌根真菌的侵染率.
In order to study the effects of AM fungi on rhizosphere soil micro-ecological environment of Citrange, the results showed that inoculation of AM fungi with Rhizoctonia solani, Glomus mosseae, The results showed that: the inoculation AM fungi formed 40% ~ 70% mycorrhizal infection rate; the mycorrhizal citrange height, the stems, aboveground / underground biomass and other vegetative growth significantly increased; AM fungi on the rhizosphere (P <0.05). The AM fungi inoculated with AM fungi increased the number of microbe population and the number of bacteria, actinomycetes, fungi, azotobacter, ammonification bacteria and cellulolytic bacteria in root zone significantly increased Soil alkaline phosphatase, urease activity, various enzyme activities and mycorrhizal infection rate was significantly positively correlated (p <0.01); rhizosphere soil inoculated with mycorrhizal can be directly absorbed by plants N, P enrichment phenomenon, and N, P content and mycorrhizal infection rate was significantly positively correlated.Path analysis showed that: rhizosphere soil citrange soil microorganisms, enzyme activity, pH and nutrients through direct and indirect effects together Affect the infection rate of mycorrhizal fungi.