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恶性胶质瘤对化疗药物敏感性的异质性是影响化疗疗效的重要因素之一。为了探讨胶质瘤细胞的染色体数目与其对化疗药物的敏感性之间是否相关,我们采用CFA测定染色体众数不同的单层培养及克隆培养的肿瘤细胞对BCNU敏感性的差别;同时对自发性及获得性抗BCNU细胞的染色体数目进行了分析。结果发现抗药细胞均为二倍体或近似二倍体细胞,而多倍体细胞则往往为药物敏感细胞。虽其机制尚待进一步澄清,但初步设想有可能近似二倍体细胞对化疗药物引起的DNA损伤修复较完善些。
The heterogeneity of glioblastoma sensitivity to chemotherapy drugs is one of the important factors affecting the efficacy of chemotherapy. To investigate whether there is a correlation between chromosome number of glioma cells and its sensitivity to chemotherapeutic drugs, we used CFA to determine the difference in BCNU sensitivity between monolayer cultures and clone cultured tumor cells with different chromosome patterns; The chromosome number of acquired anti-BCNU cells was analyzed. The results showed that the drug-resistant cells were all diploid or nearly diploid cells, while the polyploid cells were often drug-sensitive cells. Although its mechanism has yet to be further clarified, it is tentatively conceived that it is possible to approximate the repair of DNA damage by diploid cells caused by chemotherapy drugs.