SIRS与MODS患者外周血单核细胞环氧化酶与血小板活化因子的关系研究

来源 :中国危重病急救医学 | 被引量 : 0次 | 上传用户:jinger1999
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
目的了解环氧化酶(COX)和血小板活化因子(PAF)在全身炎症反应综合征(SIRS)与多器官功能障碍综合征(MODS)患者发病机制中的作用。方法选择28例符合美国胸科医师协会/危重病医学会(ACCP/SCCM)提出的SIRS和MODS诊断标准患者,其中SIRS组13例,MODS组15例,另以与患者年龄、性别相匹配的11名健康体检者作为正常对照组。采用淋巴细胞分离液密度梯度离心法分离外周血单核细胞(PBMCs);用酶联免疫吸附法(ELISA)检测PBMCs中COX2含量与血小板活化因子乙酰水解酶(PAF AH)活性;用逆转录聚合酶链反应(RT PCR)法检测PBMCs中COX2与PAF AH的mRNA表达。结果MODS组患者PBMCs中COX2含量、PAF AH活性及两者的mRNA表达均显著高于正常对照组和SIRS组(P均<0.05),正常对照组与SIRS组间差异无显著性,死亡患者高于存活患者(P均<0.05);3组PBMCs中COX2含量与PAF AH活性间呈正相关关系(r=0.329,P<0.05)。死亡患者的外周血白细胞计数、淋巴细胞计数、氧合指数与存活患者比较差异均无显著性,血糖、血肌酐则明显高于存活患者(P<0.05和P<0.01),CO2总含量(TCO2)、动脉血pH值均明显低于存活患者(P均<0.01)。结论COX2与PAF AH参与了MODS的发病过程,而且可作为判断SIRS与MODS预后的参考指标;血糖、肌酐、TCO2、pH可作为判断病情及预后的其他参考指标。 Objective To investigate the role of cyclooxygenase (COX) and platelet activating factor (PAF) in the pathogenesis of systemic inflammatory response syndrome (SIRS) and multiple organ dysfunction syndrome (MODS). Methods Twenty-eight patients with SIRS and MODS were enrolled in this study. Thirteen patients in the SIRS group and 15 patients in the MODS group were selected according to the criteria of ACCP / SCCM. The patients were matched with age and gender Eleven healthy people as normal control group. Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation of lymphocytes; COX2 content and PAF-AH activity in PBMCs were detected by enzyme-linked immunosorbent assay (ELISA) The mRNA expression of COX2 and PAF AH in PBMCs was detected by RT-PCR. Results The levels of COX2 and PAF-AH in both PBMCs and MODS were significantly higher in MODS group than those in SIRS group and normal control group (all P <0.05). There was no significant difference between SIRS group and normal control group (P <0.05). There was a positive correlation between COX2 content and PAF AH activity in three groups of PBMCs (r = 0.329, P <0.05). There were no significant differences in peripheral blood leucocyte count, lymphocyte count, oxygenation index and survivors in the patients with death, and blood glucose and serum creatinine were significantly higher than those in survivors (P <0.05 and P <0.01) ), Arterial blood pH values ​​were significantly lower than those of surviving patients (all P <0.01). Conclusions COX2 and PAF AH are involved in the pathogenesis of MODS, and can be used as a reference index to judge the prognosis of SIRS and MODS. Blood glucose, creatinine, TCO2 and pH can be used as other reference indexes to judge the disease and prognosis.
其他文献
人力资源管理对企业获取竞争优势有着重要的作用。以往的研究大都集中在对人力资源职能本身的研究上,认为人力资源是一种核心资源和能力,具有价值、稀缺性和难以模仿性,从而
目的:探讨缺氧复氧对血管内皮细胞分泌一氧化氮(NO)及NO合酶(NOS)活力的影响及辛伐他汀的干预作用,并分析其产生作用的可能机制。方法:体外培养人脐静脉内皮细胞株ECV304,使
介绍了电子秤系统的技术指标和设计考虑.
对于广西这样一个基国际金融技术相对滞后的省份来说,如何在对外开放中进行国际金融创新以推进基础设施建设及贸易与金融的同步发展呢?本文在分析现状的基础上提出了对广西国
通过对埃勒公司28位管理人员和骨干人员360°绩效考评结果的统计分析,发现不同类型考评主体的考评结果呈现不同的特征,文章进一步探讨了其原因,并为提高绩效考评的准确性和客
网络边沿缓存能够加速流媒体的传输,它是一种提高Internet上流媒体服务性能的技术。为了减少流媒体服务启动延时与抖动,降低流媒体服务器服务时的资源(CPU的利用与网络带宽)
目的:探讨医学影像学在诊断先天性联体畸形中的价值,以便做好分离术前准备.材料与方法:收集我院两例联体婴儿的消化道造影、CT、MRI、核医学等影像学资料,分析各自特异性表现
Java Application是Java程序设计的一个重要分支.如何很好地运用‘类’这种模块化的思想来开发Java Application是一个重要的话题.文中通过设计开发一个具有一定应用背景的小
随着网络技术的发展,传统的数字化办公已经越来越不能满足企业的需要了,一个可以给企业信息化管理带来高效的软件已经是每个企业所必须的了.传统的这类软件往往功能单一或功
目的 了解化学合成小干扰RNA(small interference RNA,siRNA)对原代培养神经元Nogo受体(Nogo receptor,NgR)mRNA作用的时效关系和毒性作用.方法 化学合成一段针对大鼠NgR基因