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目的 :研究SARS病毒感染后机体产生保护性免疫应答的规律和可能机制。方法 :通过生物信息学分析 ,在原核表达系统中表达了SARS病毒S1蛋白。利用SARS流行前正常人血清和 6份SARS患者恢复期的血清 ,对纯化的重组S1蛋白进行血清学分析。结果 :研究中克隆表达的重组蛋白序列与公布的SARS病毒S1蛋白的序列相同。 6份SARS患者恢复期的血清均能识别重组S1蛋白 ,而 6份SARS流行前的正常人血清则不能与重组蛋白反应。结论 :获得的重组S1蛋白具有与天然SARS病毒S1蛋白相同的序列 ,并具有与之相似的血清学反应性 ,为研究SARS病毒感染免疫应答的过程及其机制和制备SARS病毒的重组疫苗提供了良好的物质基础
Objective: To study the regularity and possible mechanism of protective immune response after SARS virus infection. Methods: SARS virus S1 protein was expressed in prokaryotic expression system by bioinformatics analysis. Purified recombinant S1 protein was serologically analyzed using serum from pre-nosocomial SARS patients and convalescent sera from 6 SARS patients. Results: The recombinant protein sequence cloned and expressed in the study was the same as the published SARS virus S1 protein sequence. The serum of all six SARS patients recovered at the same time could recognize recombinant S1 protein, whereas the normal serum of six SARS patients did not react with the recombinant protein. CONCLUSION: The recombinant S1 protein obtained has the same sequence as the S1 protein of the natural SARS virus and has similar serological reactivity. It provides a mechanism for studying the immune response against SARS virus infection and the recombinant vaccine for preparing the SARS virus Good material foundation