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目的:比较清肝利肠灌肠液合煎液与单煎液中芦荟大黄素、大黄酸、大黄素、大黄酚和大黄索甲醚的含量变化。方法:采用高效液相色谱法,色谱柱为Kromasil 100-5C_(18)(250 mm×4.6 mm,5μm),甲醇-0.1%磷酸溶液为流动相进行梯度洗脱,流速1.0 ml·min~(-1),检测波长254 nm。结果:芦荟大黄素、大黄酸、大黄素和大黄酚的在清肝利肠灌肠合煎液中的含量分别为0.172 8,0.662 5,0.233 0,0.173 9,0.052 9 mg·g~(-1);在单煎液中的含量分别为0.215 2,1.201 0,0.307 6,0.326 6,0.051 5 mg·g~(-1)。结论:芦荟大黄素、大黄酸、大黄素和大黄酚的含量清肝利肠灌肠液单煎液高于合煎液,而大黄素甲醚的含量合煎液高于单煎液。
OBJECTIVE: To compare the contents of aloe-emodin, rhein, emodin, chrysophanol and rhubarb in the combined decoction and decoction of Qinggan Li enema. Methods: High performance liquid chromatography (HPLC) was used with a gradient elution gradient of Kromasil 100-5C 18 (250 mm × 4.6 mm, 5 μm) and methanol-0.1% phosphoric acid solution as the mobile phase at a flow rate of 1.0 ml · min ~ -1), detection wavelength 254 nm. Results: The contents of aloe-emodin, rhein, emodin and chrysophanol were 0.172 8,0.662 5,0.233 0,0.173 9,0.052 9 mg · g -1 ); The content of single decoction was 0.215 2,1.201 0,0.307 6,0.326 6,0.051 5 mg · g -1, respectively. CONCLUSION: The content of aloe-emodin, rhein, emodin and chrysophanol in Qingganli enema was higher than that in combined decoction, while the content of physcion was higher than that in single decoction.