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目的:研究黄连吴茱萸药对对DMH诱导的大鼠结肠癌癌前病变以及结肠上皮增殖和凋亡的作用。方法:采用DMH制备大鼠结肠癌模型;应用形态学的方法测定经过细微分离获得的完整肠腺内细胞的增殖和凋亡变化;应用甲基绿染色和光学显微镜计数中、降结肠异变肠腺病灶数目。结果:黄连吴茱萸药对能够明显抑制模型组动物ACF的形成。模型组动物中、降结肠肠腺内细胞增殖显著增加,而黄连吴茱萸药对能够明显抑制中、降结肠肠腺内细胞的增殖;模型组动物降结肠肠腺内细胞凋亡明显增加,而中结肠肠腺内细胞凋亡较正常相比无明显差异,而黄连吴茱萸药对能够显著促进中、降结肠肠腺内细胞的凋亡。结论:黄连吴茱萸药对能够显著抑制结肠癌癌前病变ACF的形成,提示其对结肠癌可能具有抑制和临床治疗作用,其作用部分通过抑制细胞增殖和促进细胞凋亡实现。
Objective: To study the effect of Fructus Evodia on the DMH-induced colorectal cancer precancerous lesions and colon epithelial proliferation and apoptosis. Methods: The rat model of colon cancer was established by using DMH. Morphological methods were used to determine the proliferation and apoptosis of the intact glandular cells. The morphological changes of mitochondria were observed with methyl green staining and optical microscopy. Number of glandular lesions. Results: Coptis Evodia could significantly inhibit the formation of ACF in model group. In the model group, the proliferation of cells in the descending colon gland was significantly increased, whereas the Coptis chinensis fruit extract could significantly inhibit the proliferation of the cells in the middle and descending colon glands. The apoptosis in the model group decreased significantly, The apoptosis of colonic glands in the normal compared to no significant difference, while the Coptis Evodia can significantly promote the middle and lower intestinal gland cells in the apoptosis. CONCLUSION: Fructus Evodia can significantly inhibit the formation of ACF in precancerous lesions of colon cancer, suggesting that it may have an inhibitory and clinical therapeutic effect on colon cancer. Its effect may be partly through inhibiting cell proliferation and promoting apoptosis.