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目的:探讨抑癌方对小鼠结肠癌移植瘤微血管生成、血管内皮生长因子(vascular endothelial growthfactor,VEGF)及缺氧诱导因子-1α(hypoxia inducible factor-1 alpha,HIF-1α)影响。方法:用CT26结肠癌细胞建立小鼠结肠癌移植瘤模型,分阴性对照组、中药高、中、低剂量组及化疗药组5组,分别灌服生理盐水、中药及腹腔注射氟脲嘧啶(5-FU)。比较各组之间瘤重;采用免疫组化染色方法和显微图像分析技术检测不同浓度抑癌方对各组小鼠移植瘤微血管(MV)及VEGF、HIF-1a表达的影响。结果:与阴性对照组的瘤重相比较,抑癌方各组及5-FU组(阳性对照药,以下称化疗组)均有统计学差异(P<0.05);但中药各组瘤重均高于化疗组(P<0.05)。免疫组化染色结果显示阴性对照组癌巢间MV、VEGF及HIF-1a表达高于中药组和化疗组(P<0.05)。结论:抑癌方对小鼠移植瘤有一定抑制作用;能够减少肠癌移植瘤中血管形成及VEGF及HIF-1a的表达。
OBJECTIVE: To investigate the effect of Suppressant tumor suppressant on the microvessel density, vascular endothelial growth factor (VEGF) and hypoxia inducible factor-1 alpha (HIF-1α) in colon cancer xenografts in mice. Methods: The colon cancer xenografts in mice were established by CT26 colon cancer cells. The mice in each group were divided into 5 groups: normal saline, Chinese medicine, 5-FU). The tumor weight was compared between the groups. Immunohistochemical staining and microscopic image analysis were used to detect the effect of different concentrations of tumor suppressor on the microvessel density (VEGF) and the expression of VEGF and HIF-1a in the transplanted tumor. Results: Compared with the tumor weight in the negative control group, the tumor suppression group and 5-FU group (positive control drug, hereinafter referred to chemotherapy group) were statistically significant (P <0.05) Higher than chemotherapy group (P <0.05). The results of immunohistochemistry showed that the expressions of MV, VEGF and HIF-1a in the negative control group were higher than those in the traditional Chinese medicine group and the chemotherapy group (P <0.05). Conclusion: Tumor Suppressor can inhibit the transplanted tumor in mice, reduce the angiogenesis and the expression of VEGF and HIF-1a in colon cancer.